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Oogenesis

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Ioannis P. Nezis

    1. School of Life Sciences, University of Warwick, Coventry, United Kingdom

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1457)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Histological Analysis of the Arabidopsis Gynoecium and Ovules Using Chloral Hydrate Clearing and Differential Interference Contrast Light Microscopy

    • Robert G. Franks
    Pages 1-7

  3. Indirect Immunofluorescence of Proteins in Oogenic Germ Cells of Caenorhabditis elegans

    • John L. Brenner, Tim Schedl
    Pages 9-17

  4. Antibody Staining in Drosophila Germaria

    • Anette Lie-Jensen, Kaisa Haglund
    Pages 19-33

  5. In Vitro Culturing and Live Imaging of Drosophila Egg Chambers: A History and Adaptable Method

    • Nathaniel C. Peters, Celeste A. Berg
    Pages 35-68

  6. Using Fluorescent Reporters to Monitor Autophagy in the Female Germline Cells in Drosophila melanogaster

    • Anne-Claire Jacomin, Ioannis P. Nezis
    Pages 69-78

  7. Analysis of Phagocytosis in the Drosophila Ovary

    • Tracy L. Meehan, Sandy B. Serizier, Sarah E. Kleinsorge, Kimberly McCall
    Pages 79-95

  8. Immuno-Gold Labeling of Drosophila Follicles for Transmission Electron Microscopy

    • Panagiotis Tsapras, Antonia P. Sagona, Ioannis P. Nezis
    Pages 97-103

  9. Preparation of Drosophila Follicles for Transmission Electron Microscopy

    • Anthimi Palara, Antonia P. Sagona, Ioannis P. Nezis
    Pages 105-110

  10. Quantitative Real-Time PCR Analysis of Gene Transcripts of Mosquito Follicles

    • Aparna Telang
    Pages 111-127

  11. Studying Oogenesis in a Non-model Organism Using Transcriptomics: Assembling, Annotating, and Analyzing Your Data

    • Jean-Michel Carter, Melanie Gibbs, Casper J. Breuker
    Pages 129-143

  12. Live Imaging of Centriole Dynamics by Fluorescently Tagged Proteins in Starfish Oocyte Meiosis

    • Joana Borrego-Pinto, Kálmán Somogyi, Péter Lénárt
    Pages 145-166

  13. High-Pressure Freezing Electron Microscopy of Zebrafish Oocytes

    • Palsamy Kanagaraj, Dietmar Riedel, Roland Dosch
    Pages 167-178

  14. Cytoskeleton and Cytoskeleton-Bound RNA Visualization in Frog and Insect Oocytes

    • Malgorzata Kloc, Szczepan Bilinski, Jacek Z. Kubiak
    Pages 179-190

  15. RNA FISH, DNA FISH and Chromosome Painting of Chicken Oocytes

    • Silvana Guioli, Robin Lovell-Badge
    Pages 191-208

  16. Preparation of Cell Lysate from Mouse Oocytes for Western Blotting Analysis

    • Petros Marangos
    Pages 209-215

  17. Quantitative Microinjection of Morpholino Antisense Oligonucleotides into Mouse Oocytes to Examine Gene Function in Meiosis-I

    • Shoma Nakagawa, Greg FitzHarris
    Pages 217-230

  18. Monitoring Calcium Oscillations in Fertilized Mouse Eggs

    • Guillaume Halet
    Pages 231-240

  19. Live Imaging of Intracellular Dynamics During Meiotic Maturation in Mouse Oocytes

    • Shuhei Yoshida, Yogo Sakakibara, Tomoya S. Kitajima
    Pages 241-251

  20. Isolation of Mammalian Oogonial Stem Cells by Antibody-Based Fluorescence-Activated Cell Sorting

    • Deanna M. Navaroli, Jonathan L. Tilly, Dori C. Woods
    Pages 253-268
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Keywords

About this book

This volume presents methods and techniques to study oogenesis in a broad range of organisms, from plants to mammals. Oogenesis: Methods and Protocols guides readers through protocols on models of developmental biology, oogenesis in plants, worms, fruit flies, mosquitos, butterflies, starfish, zebrafish, frog, chicken and mouse. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Oogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • School of Life Sciences, University of Warwick, Coventry, United Kingdom

    Ioannis P. Nezis

Bibliographic Information

  • Book Title: Oogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Ioannis P. Nezis

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3795-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3793-6Published: 25 August 2016

  • Softcover ISBN: 978-1-4939-8141-0Published: 09 June 2018

  • eBook ISBN: 978-1-4939-3795-0Published: 24 August 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 277

  • Number of Illustrations: 50 b/w illustrations

  • Topics: Cell Biology

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