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  • © 2016

Chikungunya Virus

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1426)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xiv
  2. Clinical and Diagnostic Virology

    1. Front Matter

      Pages 1-1
    2. Evolution and Epidemiology of Chikungunya Virus

      • Giada Rossini, Maria Paola Landini, Vittorio Sambri
      Pages 3-10
    3. Molecular Epidemiology of Chikungunya Virus by Sequencing

      • Ravindran Thayan, Mohd Apandi Yusof, Zainah Saat, Shamala Devi Sekaran, Seok Mui Wang
      Pages 11-19
    4. Advanced Genetic Methodologies in Tracking Evolution and Spread of Chikungunya Virus

      • Hapuarachchige Chanditha Hapuarachchi, Kim-Sung Lee
      Pages 21-37
    5. Synthetic Peptide-Based Antibody Detection for Diagnosis of Chikungunya Infection with and without Neurological Complications

      • Rajpal S. Kashyap, Shradha S. Bhullar, Nitin H. Chandak, Girdhar M. Taori
      Pages 39-50
    6. Diagnostic Methods for CHIKV Based on Serological Tools

      • Paolo Gaibani, Maria Poala Landini, Vittorio Sambri
      Pages 63-73
  3. Cell Culture, Virus Replication, and Cellular Responses

    1. Front Matter

      Pages 85-85
    2. Propagation of Chikungunya Virus Using Mosquito Cells

      • Swee Kim Ang, Shirley Lam, Justin Jang Hann Chu
      Pages 87-92
    3. Infectious Viral Quantification of Chikungunya Virus—Virus Plaque Assay

      • Parveen Kaur, Regina Ching Hua Lee, Justin Jang Hann Chu
      Pages 93-103
    4. Detection and Quantification of Chikungunya Virus by Real-Time RT-PCR Assay

      • Seok Mui Wang, Ummul Haninah Ali, Shamala Devi Sekaran, Ravindran Thayan
      Pages 105-117
    5. Chikungunya Virus Infection of Aedes Mosquitoes

      • Hui Vern Wong, Yoke Fun Chan, I-Ching Sam, Wan Yusof Wan Sulaiman, Indra Vythilingam
      Pages 119-128
    6. Analysis of CHIKV in Mosquitoes Infected via Artificial Blood Meal

      • Jeremy P. Ledermann, Ann M. Powers
      Pages 129-142
    7. Application of GelC-MS/MS to Proteomic Profiling of Chikungunya Virus Infection: Preparation of Peptides for Analysis

      • Atchara Paemanee, Nitwara Wikan, Sittiruk Roytrakul, Duncan R. Smith
      Pages 179-193

About this book

This volume covers multidisciplinary approaches on various aspects of Chikungunya Virus (CHIKV) research that was brought together from leading laboratories across the globe. Topics covered include techniques in clinical and diagnostic virology, basic protocols in cell and virus culture, bioinformatics, and proteomics approaches in cellular response studies. Chikungunya Virus: Methods and Protocols also discusses methods in immunology and animal model studies, as well as different strategies of antivirals and vaccine development for therapeutics against CHIKV infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary material and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and practical, Chikungunya Virus: Methods and Protocols is a useful and comprehensive resource for CHIKV researchers and anyone interested in learning more about the Chikungunya Virus.

Editors and Affiliations

  • Dept of Microbiology and Immunology, National University of Singapore, Singapore, Singapore

    Justin Jang Hann Chu, Swee Kim Ang

Bibliographic Information

  • Book Title: Chikungunya Virus

  • Book Subtitle: Methods and Protocols

  • Editors: Justin Jang Hann Chu, Swee Kim Ang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3618-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3616-8Published: 28 May 2016

  • Softcover ISBN: 978-1-4939-8096-3Published: 26 May 2018

  • eBook ISBN: 978-1-4939-3618-2Published: 27 May 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 335

  • Number of Illustrations: 11 b/w illustrations, 41 illustrations in colour

  • Topics: Infectious Diseases, Virology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access