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Methods in Molecular Biology

Nonribosomal Peptide and Polyketide Biosynthesis

Methods and Protocols

Editors: Evans, Bradley S. (Ed.)

  • Includes cutting-edge methods and protocols for NPRS and PKS research
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA (gross)
  • ISBN 978-1-4939-3375-4
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-4939-3373-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume presents an overview of nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) structure and function.  It then continues with methods for the analysis of these pathways including conventional enzymological assays, contemporary mass spectrometric analysis techniques, specialized molecular biological approaches applicable to NRPSs and PKSs, and small molecule analysis tools tailored to this very special class of natural products, and concludes by examining bioinformatics tools for the analysis of these enzymes, pathways, and molecules. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Nonribosomal Peptide and Polyketide Biosynthesis: Methods and Protocols serves as a valuable reference for those experienced in studying NRPS and PKS enzymes, pathways, and natural products as well as a gateway for those just entering the field.

Table of contents (17 chapters)

  • Structural Biology of Nonribosomal Peptide Synthetases

    Miller, Bradley R. (et al.)

    Pages 3-29

  • The Assembly Line Enzymology of Polyketide Biosynthesis

    Till, Marisa (et al.)

    Pages 31-49

  • Measurement of Nonribosomal Peptide Synthetase Adenylation Domain Activity Using a Continuous Hydroxylamine Release Assay

    Duckworth, Benjamin P. (et al.)

    Pages 53-61

  • Affinity Purification Method for the Identification of Nonribosomal Peptide Biosynthetic Enzymes Using a Synthetic Probe for Adenylation Domains

    Ishikawa, Fumihiro (et al.)

    Pages 63-76

  • Colorimetric Detection of the Adenylation Activity in Nonribosomal Peptide Synthetases

    Maruyama, Chitose (et al.)

    Pages 77-84

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-4939-3375-4
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-4939-3373-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Nonribosomal Peptide and Polyketide Biosynthesis
Book Subtitle
Methods and Protocols
Editors
  • Bradley S. Evans
Series Title
Methods in Molecular Biology
Series Volume
1401
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3375-4
DOI
10.1007/978-1-4939-3375-4
Hardcover ISBN
978-1-4939-3373-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 281
Number of Illustrations and Tables
49 b/w illustrations, 31 illustrations in colour
Topics