Editors:
- Includes cutting-edge methods and protocols for NPRS and PKS research
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1401)
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Table of contents (17 protocols)
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Front Matter
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Background and Overview
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Front Matter
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In Vitro Methods
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Front Matter
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Bioinformatic Methods
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Front Matter
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About this book
This volume presents an overview of nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) structure and function. It then continues with methods for the analysis of these pathways including conventional enzymological assays, contemporary mass spectrometric analysis techniques, specialized molecular biological approaches applicable to NRPSs and PKSs, and small molecule analysis tools tailored to this very special class of natural products, and concludes by examining bioinformatics tools for the analysis of these enzymes, pathways, and molecules. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Nonribosomal Peptide and Polyketide Biosynthesis: Methods and Protocols serves as a valuable reference for those experienced in studying NRPS and PKS enzymes, pathways, and natural products as well as a gateway for those just entering the field.
Editors and Affiliations
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Donald Danforth Plant Science Center, Saint Louis, USA
Bradley S. Evans
Bibliographic Information
Book Title: Nonribosomal Peptide and Polyketide Biosynthesis
Book Subtitle: Methods and Protocols
Editors: Bradley S. Evans
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-3375-4
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-3373-0Published: 02 February 2016
Softcover ISBN: 978-1-4939-8034-5Published: 31 March 2018
eBook ISBN: 978-1-4939-3375-4Published: 02 February 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 281
Number of Illustrations: 31 b/w illustrations, 49 illustrations in colour
Topics: Protein Science, Enzymology