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Methods in Molecular Biology

The Genetic Manipulation of Staphylococci

Methods and Protocols

Editors: Bose, Jeffrey L. (Ed.)

  • Includes cutting-edge methods and protocols for the study of Staphylococci genetic manipulation
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA (gross)
  • ISBN 978-1-4939-3158-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-4939-3157-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This detailed book contains chapters using unbiased approaches such as chemical and transposon mutagenesis, as well as a protocol for allelic exchange, to make targeted mutants in Staphylococcus, as generating mutants of this bacteria can be accomplished in many ways, depending on the intended changes. Using these methods, the authors have been successful at making mutants that span individual single-nucleotide changes in the chromosome to whole genome mutant libraries. The latter strategy provides a useful tool for high-throughput screening, while single nucleotide changes are an elegant way of teasing apart the importance of single nucleotides in gene expression or to create specific amino acid substitutions to examine protein function. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, The Genetic Manipulation of Staphylococci: Methods and Protocols serves as a vital resource and guide to scientists in the Staphylococcus community as they pursue their studies on these bacteria.

Table of contents (20 chapters)

  • Understanding Staphylococcal Nomenclature

    Krute, Christina N. (et al.)

    Pages 1-7

  • Restriction–Modification Systems as a Barrier for Genetic Manipulation of Staphylococcus aureus

    Sadykov, Marat R.

    Pages 9-23

  • Complementation Plasmids, Inducible Gene-Expression Systems, and Reporters for Staphylococci

    Bertram, Ralph

    Pages 25-32

  • De Novo Assembly of Plasmids Using Yeast Recombinational Cloning

    Mashruwala, Ameya A. (et al.)

    Pages 33-41

  • Splicing by Overlap Extension PCR to Obtain Hybrid DNA Products

    Thornton, Justin A.

    Pages 43-49

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-4939-3158-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-4939-3157-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
The Genetic Manipulation of Staphylococci
Book Subtitle
Methods and Protocols
Editors
  • Jeffrey L. Bose
Series Title
Methods in Molecular Biology
Series Volume
1373
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3158-3
DOI
10.1007/978-1-4939-3158-3
Hardcover ISBN
978-1-4939-3157-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 186
Number of Illustrations and Tables
16 b/w illustrations, 7 illustrations in colour
Topics