Methods in Molecular Biology

Estrogen Receptors

Methods and Protocols

Editors: Eyster, Kathleen M. (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $119.00
price for USA (gross)
  • ISBN 978-1-4939-3127-9
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  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.00
price for USA
  • ISBN 978-1-4939-3126-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $159.00
price for USA
  • ISBN 978-1-4939-5006-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume examines a wide array of vital technologies for advancing our understanding of the receptor-mediated actions of estrogen. The protocols in this book range from standard methods and vital laboratory workhorses, such as receptor binding assays and western blot, to newer technologies such as RNAseq and proximity ligation assay. Chapters also discuss protocols from a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Estrogen Receptors: Methods and Protocols is a detailed and helpful resource for scientists who are intrigued by the many facets of estrogen.

The chapter 'Bioinformatics Analysis of Estrogen-Responsive Genes' is open access under a CC BY 4.0 license. 

About the authors

Kathleen (Kathy) Eyster Professor of Physiology and Pharmacology Sanford School of Medicine of the University of South Dakota 605-677-5159

Table of contents (43 chapters)

  • The Estrogen Receptors: An Overview from Different Perspectives

    Eyster, Kathleen M.

    Pages 1-10

  • Competitive Binding Assay for the G-Protein-Coupled Receptor 30 (GPR30) or G-Protein-Coupled Estrogen Receptor (GPER)

    Thekkumkara, Thomas (et al.)

    Pages 11-17

  • The Use of Real-Time Reverse Transcription-PCR for Assessing Estrogen Receptor and Estrogen-Responsive Gene Expression

    Booze, Michelle L. (et al.)

    Pages 19-28

  • Bioinformatics Analysis of Estrogen-Responsive Genes

    Handel, Adam E.

    Pages 29-39

  • Electrophoretic Mobility Shift Assay (EMSA) and Supershift Assay of Cytochrome P450 2B6 in Response to Estrogen

    Koh, Kwi Hye (et al.)

    Pages 41-51

Buy this book

eBook $119.00
price for USA (gross)
  • ISBN 978-1-4939-3127-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.00
price for USA
  • ISBN 978-1-4939-3126-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $159.00
price for USA
  • ISBN 978-1-4939-5006-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Estrogen Receptors
Book Subtitle
Methods and Protocols
Editors
  • Kathleen M. Eyster
Series Title
Methods in Molecular Biology
Series Volume
1366
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3127-9
DOI
10.1007/978-1-4939-3127-9
Hardcover ISBN
978-1-4939-3126-2
Softcover ISBN
978-1-4939-5006-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 576
Number of Illustrations and Tables
41 b/w illustrations, 86 illustrations in colour
Topics