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HIV Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Vinayaka R. Prasad

    1. Department of Microbiology & Immunology, Albert Einstein College of Medicine, BRONX, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Ganjam V. Kalpana

    1. Department of Genetics, Albert Einstein College of Medicine, BRONX, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1354)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. HIV-1 Replication—Early Events

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Quantifying CD4/CCR5 Usage Efficiency of HIV-1 Env Using the Affinofile System

      • Nicholas E. Webb, Benhur Lee
      Pages 3-20

    3. Measuring T Cell-to-T Cell HIV-1 Transfer, Viral Fusion, and Infection Using Flow Cytometry

      • Natasha D. Durham, Benjamin K. Chen
      Pages 21-38

    4. HIV-1 Capsid Stabilization Assay

      • Thomas Fricke, Felipe Diaz-Griffero
      Pages 39-47

    5. Detection and Tracking of Dual-Labeled HIV Particles Using Wide-Field Live Cell Imaging to Follow Viral Core Integrity

      • João I. Mamede, Thomas J. Hope
      Pages 49-59

    6. HIV-1 Reverse Transcriptase-Based Assay to Determine Cellular dNTP Concentrations

      • Joseph A. Hollenbaugh, Baek Kim
      Pages 61-70

    7. Rapid Determination of HIV-1 Mutant Frequencies and Mutation Spectra Using an mCherry/EGFP Dual-Reporter Viral Vector

      • Jonathan M. O. Rawson, Christine L. Clouser, Louis M. Mansky
      Pages 71-88

  3. HIV-1 RNA Structure and RNA-Protein Interactions

    1. Front Matter

      Pages 89-89
      Download chapter PDF

    2. Novel Biochemical Tools for Probing HIV RNA Structure

      • Jason W. Rausch, Joanna Sztuba-Solinska, Sabrina Lusvarghi, Stuart F. J. Le Grice
      Pages 91-117

    3. Analysis of HIV-1 Gag-RNA Interactions in Cells and Virions by CLIP-seq

      • Sebla B. Kutluay, Paul D. Bieniasz
      Pages 119-131

    4. Isolation of Cognate Cellular and Viral Ribonucleoprotein Complexes of HIV-1 RNA Applicable to Proteomic Discovery and Molecular Investigations

      • Deepali Singh, Ioana Boeras, Gatikrushna Singh, Kathleen Boris-Lawrie
      Pages 133-146

  4. HIV-1 Replication—Post-integration Events

    1. Front Matter

      Pages 147-147
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    2. Methods for the Analyses of Inhibitor-Induced Aberrant Multimerization of HIV-1 Integrase

      • Jacques J. Kessl, Amit Sharma, Mamuka Kvaratskhelia
      Pages 149-164

    3. Quantification of HIV-1 Gag Localization Within Virus Producer Cells

      • Annalena La Porte, Ganjam V. Kalpana
      Pages 165-174

    4. Methods to Study Determinants for Membrane Targeting of HIV-1 Gag In Vitro

      • Gabrielle C. Todd, Akira Ono
      Pages 175-185

  5. Studying HIV-1 Replication and Pathogenesis in Animal Models

    1. Front Matter

      Pages 187-187
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    2. Visualizing the Behavior of HIV-Infected T Cells In Vivo Using Multiphoton Intravital Microscopy

      • Radwa Sharaf, Thorsten R. Mempel, Thomas T. Murooka
      Pages 189-201

    3. Modeling HIV-1 Mucosal Transmission and Prevention in Humanized Mice

      • Milena Veselinovic, Paige Charlins, Ramesh Akkina
      Pages 203-220

    4. High-Throughput Humanized Mouse Models for Evaluation of HIV-1 Therapeutics and Pathogenesis

      • Tynisha Thomas, Kieran Seay, Jian Hua Zheng, Cong Zhang, Christina Ochsenbauer, John C. Kappes et al.
      Pages 221-235
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Keywords

About this book

This third edition volume expands upon the previous editions with new research topics and numerous leading-edge HIV protocols. This book contains chapters that cover various aspects of HIV-1 replication and are helpful for investigating the mechanisms of HIV-1 replication or pathogenesis. The chapters are grouped into six sections: HIV early events; HIV-1 RNA structure and RNA-protein interactions; HIV-1 post-integration events; HIV-1 pathogenesis in animal models; tools to study HIV-1 latency; and Neuro AIDS. Chapters are written by research experts who have diligently developed techniques, improved them, simplified them, or applied them to their own HIV research. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, HIV Protocols, Third Edition is a great resource for HIV research, and contains many significant tips and tricks rarely mentioned in standard protocols.

Editors and Affiliations

  • Department of Microbiology & Immunology, Albert Einstein College of Medicine, BRONX, USA

    Vinayaka R. Prasad

  • Department of Genetics, Albert Einstein College of Medicine, BRONX, USA

    Ganjam V. Kalpana

Bibliographic Information

  • Book Title: HIV Protocols

  • Editors: Vinayaka R. Prasad, Ganjam V. Kalpana

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3046-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3045-6Published: 29 December 2015

  • Softcover ISBN: 978-1-4939-7979-0Published: 27 March 2019

  • eBook ISBN: 978-1-4939-3046-3Published: 29 December 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XIV, 383

  • Number of Illustrations: 27 b/w illustrations, 36 illustrations in colour

  • Topics: Immunology, Virology

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