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  • © 2015

Drosophila Oogenesis

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1328)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
  2. Drosophila melanogaster Oogenesis: An Overview

    • John M. McLaughlin, Diana P. Bratu
    Pages 1-20
  3. Basic Techniques in Drosophila Ovary Preparation

    • Letitia Thompson, Kristen Randolph, Amanda Norvell
    Pages 21-28
  4. Mosaic Analysis in the Drosophila melanogaster Ovary

    • Thomas Rubin, Jean-René Huynh
    Pages 29-55
  5. Genetic Mosaic Analysis of Stem Cell Lineages in the Drosophila Ovary

    • Kaitlin M. Laws, Daniela Drummond-Barbosa
    Pages 57-72
  6. Border Cell Migration: A Model System for Live Imaging and Genetic Analysis of Collective Cell Movement

    • Mohit Prasad, Xiaobo Wang, Li He, Danfeng Cai, Denise J. Montell
    Pages 89-97
  7. Visualizing Microtubule Networks During Drosophila Oogenesis Using Fixed and Live Imaging

    • Kevin Legent, Nicolas Tissot, Antoine Guichet
    Pages 99-112
  8. Single-Molecule RNA In Situ Hybridization (smFISH) and Immunofluorescence (IF) in the Drosophila Egg Chamber

    • Livia V. Bayer, Mona Batish, Stephen K. Formel, Diana P. Bratu
    Pages 125-136
  9. Fluorescent In Situ Hybridization of Nuclear Bodies in Drosophila melanogaster Ovaries

    • Zehra F. Nizami, Ji-Long Liu, Joseph G. Gall
    Pages 137-149
  10. Ultrastructural Analysis of Drosophila Ovaries by Electron Microscopy

    • Thomas R. Hurd, Carlos G. Sanchez, Felipe K. Teixeira, Chris Petzold, Kristen Dancel-Manning, Ju-Yu S. Wang et al.
    Pages 151-162
  11. Immuno-Electron Microscopy and Electron Microscopic In Situ Hybridization for Visualizing piRNA Biogenesis Bodies in Drosophila Ovaries

    • Shinsuke Shibata, Yukiko Murota, Yoshinori Nishimoto, Mana Yoshimura, Toshihiro Nagai, Hideyuki Okano et al.
    Pages 163-178
  12. Detection of Cell Death and Phagocytosis in the Drosophila Ovary

    • Tracy L. Meehan, Alla Yalonetskaya, Tony F. Joudi, Kimberly McCall
    Pages 191-206
  13. Analysis of Cell Cycle Switches in Drosophila Oogenesis

    • Dongyu Jia, Yi-Chun Huang, Wu-Min Deng
    Pages 207-216
  14. Back Matter

    Pages 243-245

About this book

This volume provides current up-to-date protocols for preparing the ovary for various imaging techniques, genetic protocols for generating mutant clones, mosaic analysis and assessing cell death. Chapters address methods for performing genome wide gene expression analysis and bioinformatics for studies of RNA-protein interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Drosophila Oogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Biological Sciences Department, Hunter College, New York, USA

    Diana P. Bratu

  • School of Arts and Sciences, Department of Biology,, Jamaica, USA

    Gerard P. McNeil

Bibliographic Information

  • Book Title: Drosophila Oogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Diana P. Bratu, Gerard P. McNeil

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2851-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2850-7Published: 01 September 2015

  • Softcover ISBN: 978-1-4939-4933-5Published: 22 October 2016

  • eBook ISBN: 978-1-4939-2851-4Published: 31 August 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 245

  • Number of Illustrations: 22 b/w illustrations, 29 illustrations in colour

  • Topics: Cell Biology, Animal Models

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access