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Methods in Molecular Biology

Affinity Chromatography

Methods and Protocols

Editors: Reichelt, Senta (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step details essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA (gross)
  • ISBN 978-1-4939-2447-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-4939-2446-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

The aim of this edition is to introduce the beginner to the basics of affinity chromatography and provide practical knowledge for the development of affinity separation protocols. Affinity Chromatography: Methods and Protocols, Third Edition guides readers through new state of the art protocols, molecular modelling, and the study of ligand-target interactions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Affinity Chromatography: Methods and Protocols, Third Edition is designed as a useful resource for those interested in the rapid and quantitative isolation of biomolecules with high purity.

Table of contents (26 chapters)

  • Affinity Chromatography: A Historical Perspective

    Hage, David S. (et al.)

    Pages 1-19

  • Protein Purification by Aminosquarylium Cyanine Dye-Affinity Chromatography

    Graça, Vânia C. (et al.)

    Pages 23-33

  • One-Step Purification of Phosphinothricin Acetyltransferase Using Reactive Dye-Affinity Chromatography

    Wang, Cunxi (et al.)

    Pages 35-42

  • Antibody Purification from Human Plasma by Metal-Chelated Affinity Membranes

    Yavuz, Handan (et al.)

    Pages 43-46

  • Specific Recognition of Supercoiled Plasmid DNA by Affinity Chromatography Using a Synthetic Aromatic Ligand

    Caramelo-Nunes, Catarina (et al.)

    Pages 47-54

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-4939-2447-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-4939-2446-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Affinity Chromatography
Book Subtitle
Methods and Protocols
Editors
  • Senta Reichelt
Series Title
Methods in Molecular Biology
Series Volume
1286
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2447-9
DOI
10.1007/978-1-4939-2447-9
Hardcover ISBN
978-1-4939-2446-2
Series ISSN
1064-3745
Edition Number
3
Number of Pages
XIII, 341
Number of Illustrations and Tables
41 b/w illustrations, 37 illustrations in colour
Topics