175 years of Springer publishing +++ Through June 30: 50% off Physics & Astronomy Books

Methods in Molecular Biology

Plant Functional Genomics

Methods and Protocols

Editors: Alonso, Jose M., Stepanova, Anna N. (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
see more benefits

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-4939-2444-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-4939-2443-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-4913-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This second edition volume discusses the revolutionary development of faster and less expensive DNA sequencing technologies from the past 10 years and focuses on general technologies that can be utilized by a wide array of plant biologists to address specific questions in their favorite model systems. This book is organized into five parts. Part I examines the tools and methods required for identifying epigenetic and conformational changes at the whole-genome level. Part II presents approaches used to determine key aspects of a gene’s function, such as techniques used to identify and characterize gene regulatory networks. This is followed by a discussion of tools used to analyze the levels of mRNA, mRNA translation rates and metabolites. Part III features a compilation of forward and reverse genetic approaches that include recent implementation of high-throughput sequencing in classical methodologies such as QTL mapping. The final two parts explore strategies to facilitate and accelerate the generation and testing of functional DNA elements and basic computational tools used to facilitate the use of systems biology approached by a broad spectrum of plant researchers. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Practical and timely, Plant Functional Genomics: Methods and Protocols, Second Edition highlights the latest developments in DNA sequencing technologies that are likely to continue shaping the future of functional genomics.

Table of contents (25 chapters)

  • Epigenome Profiling of Specific Plant Cell Types Using a Streamlined INTACT Protocol and ChIP-seq

    Wang, Dongxue (et al.)

    Pages 3-25

  • Whole-Genome DNA Methylation Profiling with Nucleotide Resolution

    Hsieh, Tzung-Fu

    Pages 27-40

  • High-Throughput Nuclease-Mediated Probing of RNA Secondary Structure in Plant Transcriptomes

    Vandivier, Lee E. (et al.)

    Pages 41-70

  • Genome-Wide Mapping of DNase I Hypersensitive Sites in Plants

    Zhang, Wenli (et al.)

    Pages 71-89

  • Characterization of In Vivo DNA-Binding Events of Plant Transcription Factors by ChIP-seq: Experimental Protocol and Computational Analysis

    Mourik, Hilda (et al.)

    Pages 93-121

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-4939-2444-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-4939-2443-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-4913-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Plant Functional Genomics
Book Subtitle
Methods and Protocols
Editors
  • Jose M. Alonso
  • Anna N. Stepanova
Series Title
Methods in Molecular Biology
Series Volume
1284
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2444-8
DOI
10.1007/978-1-4939-2444-8
Hardcover ISBN
978-1-4939-2443-1
Softcover ISBN
978-1-4939-4913-7
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XV, 532
Number of Illustrations and Tables
21 b/w illustrations, 64 illustrations in colour
Topics