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Methods in Molecular Biology

Gene Essentiality

Methods and Protocols

Editors: Lu, Long Jason (Ed.)

  • Includes cutting-edge methods and protocols for the study of microbial gene essentiality
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA (gross)
  • ISBN 978-1-4939-2398-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-4939-2397-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-4862-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and Candida albicans. Given the significant advancement in the computational predictions of microbial essential genes, the second half of the book examines four main types of approaches: comparative genomics, supervised machine learning, constraint-based methods, and corrections of transposon mutagenesis data, as well as databases and servers that are often used in studying gene essentiality. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Gene Essentiality: Methods and Protocols will aid researchers who wish to further our knowledge in this vital field of study.

Table of contents (15 chapters)

  • Microarray Transposon Tracking for the Mapping of Conditionally Essential Genes in Campylobacter jejuni

    Stahl, Martin (et al.)

    Pages 1-14

  • Identifying Essential Streptococcus sanguinis Genes Using Genome-Wide Deletion Mutation

    Chen, Lei (et al.)

    Pages 15-23

  • Defining Essential Genes and Identifying Virulence Factors of Porphyromonas gingivalis by Massively Parallel Sequencing of Transposon Libraries (Tn-seq)

    Klein, Brian A. (et al.)

    Pages 25-43

  • Identification of Essential Genes and Synthetic Lethal Gene Combinations in Escherichia coli K-12

    Mori, Hirotada (et al.)

    Pages 45-65

  • Identification of Genes Essential for Leptospirosis

    Bartpho, Thanatchaporn (et al.)

    Pages 67-77

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-4939-2398-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-4939-2397-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-4862-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Gene Essentiality
Book Subtitle
Methods and Protocols
Editors
  • Long Jason Lu
Series Title
Methods in Molecular Biology
Series Volume
1279
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2398-4
DOI
10.1007/978-1-4939-2398-4
Hardcover ISBN
978-1-4939-2397-7
Softcover ISBN
978-1-4939-4862-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 248
Number of Illustrations and Tables
27 b/w illustrations, 15 illustrations in colour
Topics