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Neuromethods

In Situ Hybridization Methods

Editors: Hauptmann, Giselbert (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $119.00
price for USA
  • ISBN 978-1-4939-2303-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.00
price for USA
  • ISBN 978-1-4939-2302-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $159.00
price for USA
  • ISBN 978-1-4939-4463-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications. The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.

Essential and authoritative, In Situ Hybridization Methods provides detailed protocols for newcomers to ISH, and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications.

Table of contents (30 chapters)

  • Optimization of In Situ Hybridization and Co-expression Analysis in the Central Nervous System

    Mezache, Louisa (et al.)

    Pages 3-25

  • In Situ Hybridization Methods for RNA Visualization in C. elegans

    Broitman-Maduro, Gina (et al.)

    Pages 29-44

  • Multicolored Visualization of Transcript Distributions in Drosophila Embryos

    Söll, Iris (et al.)

    Pages 45-59

  • Exploring Brain Genoarchitecture by Single and Double Chromogenic In Situ Hybridization (ISH) and Immunohistochemistry (IHC) in Whole-Mount Embryos

    Ferran, José Luis (et al.)

    Pages 61-82

  • Exploring Brain Genoarchitecture by Single and Double Chromogenic In Situ Hybridization (ISH) and Immunohistochemistry (IHC) on Cryostat, Paraffin, or Floating Sections

    Ferran, José Luis (et al.)

    Pages 83-107

Buy this book

eBook $119.00
price for USA
  • ISBN 978-1-4939-2303-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.00
price for USA
  • ISBN 978-1-4939-2302-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $159.00
price for USA
  • ISBN 978-1-4939-4463-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
In Situ Hybridization Methods
Editors
  • Giselbert Hauptmann
Series Title
Neuromethods
Series Volume
99
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2303-8
DOI
10.1007/978-1-4939-2303-8
Hardcover ISBN
978-1-4939-2302-1
Softcover ISBN
978-1-4939-4463-7
Series ISSN
0893-2336
Edition Number
1
Number of Pages
XX, 592
Number of Illustrations and Tables
15 b/w illustrations, 94 illustrations in colour
Topics