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Methods in Molecular Biology

Bacterial Pangenomics

Methods and Protocols

Editors: Mengoni, Alessio, Galardini, Marco, Fondi, Marco (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA (gross)
  • ISBN 978-1-4939-1720-4
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  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-4939-1719-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
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  • ISBN 978-1-4939-5547-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Bacterial genomics is a mature research interdisciplinary field, which is approached by ecologists, geneticists, bacteriologists, molecular biologists and evolutionary biologists working in medical, industrial and basic science. Thanks to the large diffusion of bacterial genome analysis, Bacterial Pangenomics: Methods and Protocols is able to provide the most recent methodologies about the study of bacterial pangenomes by covering the three major areas: the experimental methods for approaching bacterial pangenomics, the bio informatic pipelines for analysis and annotation of sequence data and finally the methods for inferring functional and evolutionary features from the pangenome. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Bacterial Pangenomics: Methods and Protocols will serve as a field guide for both qualified bacterial genomics investigators who want to update their technical knowledge, for less experienced researchers who want to start working with bacterial genomics and pangenomics, as well as serving as a manual and supplemental textbook for graduate students of genomics and bioinformatics.

Table of contents (19 chapters)

  • Pulsed Field Gel Electrophoresis and Genome Size Estimates

    Alduina, Rosa (et al.)

    Pages 1-14

  • Comparative Analyses of Extrachromosomal Bacterial Replicons, Identification of Chromids, and Experimental Evaluation of Their Indispensability

    Dziewit, Lukasz (et al.)

    Pages 15-29

  • Choice of Next-Generation Sequencing Pipelines

    Chierico, F. (et al.)

    Pages 31-47

  • The Pyrosequencing Protocol for Bacterial Genomes

    Rizzi, Ermanno

    Pages 49-75

  • Bacterial Metabarcoding by 16S rRNA Gene Ion Torrent Amplicon Sequencing

    Fantini, Elio (et al.)

    Pages 77-90

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-4939-1720-4
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-4939-1719-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-5547-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Bacterial Pangenomics
Book Subtitle
Methods and Protocols
Editors
  • Alessio Mengoni
  • Marco Galardini
  • Marco Fondi
Series Title
Methods in Molecular Biology
Series Volume
1231
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-1720-4
DOI
10.1007/978-1-4939-1720-4
Hardcover ISBN
978-1-4939-1719-8
Softcover ISBN
978-1-4939-5547-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 298
Number of Illustrations and Tables
17 b/w illustrations, 42 illustrations in colour
Topics