Methods in Molecular Biology

Agrobacterium Protocols

Volume 1

Editors: Wang, Kan (Ed.)

  • Emphasizes agricultural crops or plant species with economic values
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
see more benefits

Buy this book

eBook n/a
  • ISBN 978-1-4939-1695-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
Hardcover n/a
  • ISBN 978-1-4939-1694-8
  • Free shipping for individuals worldwide
Softcover n/a
  • ISBN 978-1-4939-4541-2
  • Free shipping for individuals worldwide
About this book

Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included.

Volume 1 details updated techniques available for 18 plant species drawn from cereal crops, legume plants, vegetable plants, and three model plant species: Brachypodium distachyon, Medicago truncatula, and Setaria viridis. It also updates a chapter for vector construction, a step critical to a successful plant transformation process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Agrobacterium Protocols, Third Edition facilitates the transfer of this rapidly developing technology to all researchers for use in both fundamental and applied biology.

Table of contents (29 chapters)

  • Gateway®-Compatible Plant Transformation Vectors

    Smedley, Mark A. (et al.)

    Pages 3-16

  • Brachypodium distachyon

    Bragg, Jennifer N. (et al.)

    Pages 17-33

  • Medicago truncatula Transformation Using Cotyledonary Explants

    Wright, Elane (et al.)

    Pages 35-41

  • Medicago truncatula Transformation Using Leaf Explants

    Cosson, Viviane (et al.)

    Pages 43-56

  • Setaria viridis

    Eck, Joyce (et al.)

    Pages 57-67

Buy this book

eBook n/a
  • ISBN 978-1-4939-1695-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
Hardcover n/a
  • ISBN 978-1-4939-1694-8
  • Free shipping for individuals worldwide
Softcover n/a
  • ISBN 978-1-4939-4541-2
  • Free shipping for individuals worldwide
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Agrobacterium Protocols
Book Subtitle
Volume 1
Editors
  • Kan Wang
Series Title
Methods in Molecular Biology
Series Volume
1223
Copyright
2015
Publisher
Springer-Verlag New York
Copyright Holder
Springer Science+Business Media New York
Distribution Rights
Distribution rights for India: Delhi Book Store, New Delhi, India
eBook ISBN
978-1-4939-1695-5
DOI
10.1007/978-1-4939-1695-5
Hardcover ISBN
978-1-4939-1694-8
Softcover ISBN
978-1-4939-4541-2
Series ISSN
1064-3745
Edition Number
3
Number of Pages
XIV, 368
Number of Illustrations and Tables
13 b/w illustrations, 29 illustrations in colour
Topics