Methods in Molecular Biology

T-Helper Cells

Methods and Protocols

Editors: Waisman, Ari, Becher, Burkhard (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA (gross)
  • ISBN 978-1-4939-1212-4
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-4939-1211-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-4645-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

T-Helper Cells: Methods and Protocols presents a broad selection of cutting edge protocols that will enable the reader to capture the unique features of TH cells with tools developed for the isolation of TH cells from various tissues and subsequent analysis of their functional properties in vitro, ex vivo and in vivo. Chapters cover methods of isolating T cells from various tissues in mice, protocols for the analysis of T cell function and phenotype using various cutting edge technologies, methods allowing for the manipulation of T cell function in vitro and in vivo and in vivo models of diseases in which T cells play a central role in the pathogenesis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, T-Helper Cells: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further the study of this amazingly versatile and potent cell type.

Reviews

From the book reviews:

“This is a useful source of current protocols designed to capture the unique features of T helper cells with tools developed for the isolation of these cells from various tissues and subsequent analysis of their functional properties in vitro, ex vivo, and in vivo. … This book is intended for all interested in learning about protocols and methods in T helper cells. Professional laboratory scientists as well as beginners in the field of immunology will find this book extremely useful.” (Omer Iqbal, Doody’s Book Reviews, January, 2015)

Table of contents (19 chapters)

  • Isolation of T Cells from the Skin

    Lorenz, Beate (et al.)

    Pages 3-13

  • Isolation of Leukocytes from Mouse Central Nervous System

    Lelios, Iva (et al.)

    Pages 15-19

  • Isolation of T Cells from the Gut

    Reißig, Sonja (et al.)

    Pages 21-25

  • T Cell Isolation from Mouse Kidneys

    Ludwig-Portugall, Isis (et al.)

    Pages 27-35

  • Intracellular Staining for Cytokines and Transcription Factors

    Mair, Florian (et al.)

    Pages 39-49

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-4939-1212-4
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-4939-1211-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-4645-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
T-Helper Cells
Book Subtitle
Methods and Protocols
Editors
  • Ari Waisman
  • Burkhard Becher
Series Title
Methods in Molecular Biology
Series Volume
1193
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-1212-4
DOI
10.1007/978-1-4939-1212-4
Hardcover ISBN
978-1-4939-1211-7
Softcover ISBN
978-1-4939-4645-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 229
Number of Illustrations and Tables
14 b/w illustrations, 24 illustrations in colour
Topics