Methods in Molecular Biology

Metabolic Flux Analysis

Methods and Protocols

Editors: Krömer, Jens O., Nielsen, Lars, Blank, Lars M. (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
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  • ISBN 978-1-4939-1170-7
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Hardcover $119.00
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  • ISBN 978-1-4939-1169-1
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Softcover $119.00
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  • ISBN 978-1-4939-4159-9
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About this book

Metabolic Flux Analysis: Methods and Protocols opens up the field of metabolic flux analysis to those who want to start a new flux analysis project but are overwhelmed by the complexity of the approach. Metabolic flux analysis emerged from the current limitation for the prediction of metabolic fluxes from a measured inventory of the cell. Divided into convenient thematic parts, topics in this essential volume include the fundamental characteristics of the underlying networks, the application of quantitative metabolite data and thermodynamic principles to constrain the solution space for flux balance analysis (FBA), the experimental toolbox to conduct different types of flux analysis experiments, the processing of data from 13C experiments and three chapters that summarize some recent key findings. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Metabolic Flux Analysis: Methods and Protocols presents protocols that cover a range of relevant organisms currently used in the field, providing a solid basis to anybody interested in the field of metabolic flux analysis.

Table of contents (17 chapters)

  • Stoichiometric Modelling of Microbial Metabolism

    Kuepfer, Lars

    Pages 3-18

  • Tapping the Wealth of Microbial Data in High-Throughput Metabolic Model Reconstruction

    Colasanti, Ric (et al.)

    Pages 19-45

  • Constraining the Flux Space Using Thermodynamics and Integration of Metabolomics Data

    Soh, Keng Cher (et al.)

    Pages 49-63

  • NExT: Integration of Thermodynamic Constraints and Metabolomics Data into a Metabolic Network

    Martínez, Verónica Sofía (et al.)

    Pages 65-78

  • Customization of 13C-MFA Strategy According to Cell Culture System

    Quek, Lake-Ee (et al.)

    Pages 81-90

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-4939-1170-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-4939-1169-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-4159-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Metabolic Flux Analysis
Book Subtitle
Methods and Protocols
Editors
  • Jens O. Krömer
  • Lars Nielsen
  • Lars M. Blank
Series Title
Methods in Molecular Biology
Series Volume
1191
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-1170-7
DOI
10.1007/978-1-4939-1170-7
Hardcover ISBN
978-1-4939-1169-1
Softcover ISBN
978-1-4939-4159-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 316
Number of Illustrations and Tables
19 b/w illustrations, 51 illustrations in colour
Topics