Methods in Molecular Biology

Regulatory B Cells

Methods and Protocols

Editors: Vitale, Gaetano, Mion, Francesca (Eds.)

  • Includes cutting-edge methods and protocols involving B cells with suppressive functions and their immunological properties
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA (gross)
  • ISBN 978-1-4939-1161-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-4939-1160-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-4677-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Regulatory B Cells: Methods and Protocols present the current experimental set-ups and methodologies useful for the identification and characterization of B cells with suppressive functions and for the study of their biological and immunological properties. Organized into four sections, this detailed volume covers the basic methods for the isolation and immunophenotypical characterization of specific B cell subsets from mouse and human tissues, methods for the investigation of the mechanisms of immune suppression operated by B cells, several experimental approaches for the ex vivo generation/expansion of IL-10-producing B cells, as well as procedures for the study of the immune suppressive function of B cells in specific pathological settings. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Regulatory B Cells: Methods and Protocols serves as an ideal guide for immunologists as well as for cell and molecular biologists interested in the intricacies of B cell biology.

Table of contents (19 chapters)

  • Purification and Immunophenotypic Characterization of Murine MZ and T2-MZP Cells

    Rosado, M. Manuela (et al.)

    Pages 3-16

  • Purification and Immune Phenotyping of B-1 Cells from Body Cavities of Mice

    Yenson, Vanessa (et al.)

    Pages 17-34

  • Purification and Immunophenotypic Characterization of Murine B10 B Cells

    Hong, Chao (et al.)

    Pages 35-44

  • Purification and Immunophenotypic Characterization of Human B Cells with Regulatory Functions

    Masson, Adèle (et al.)

    Pages 45-52

  • IL-10 Detection in Murine B Cells: Pros and Cons of the Different Techniques

    Lin, Xiang (et al.)

    Pages 55-69

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-4939-1161-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-4939-1160-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-4677-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Regulatory B Cells
Book Subtitle
Methods and Protocols
Editors
  • Gaetano Vitale
  • Francesca Mion
Series Title
Methods in Molecular Biology
Series Volume
1190
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-1161-5
DOI
10.1007/978-1-4939-1161-5
Hardcover ISBN
978-1-4939-1160-8
Softcover ISBN
978-1-4939-4677-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 292
Number of Illustrations and Tables
34 b/w illustrations, 19 illustrations in colour
Topics