Methods in Molecular Biology

Plant MAP Kinases

Methods and Protocols

Editors: Komis, George, Šamaj, Jozef (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
see more benefits

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-4939-0922-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-4939-0921-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-4589-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Mitogen-activated protein kinases (MAPKs) are versatile phosphorylating enzymes which regulate multiple proteins involved in gene expression, cell architecture, plant development and reaction to diverse abiotic and biotic factors. The main aim of Plant MAP Kinases: Methods and Protocols is to provide established and new MAPK protocols adapted to the challenges posed by working with plants. The book contains 19 chapters which encompass a wide array of methods progressively scaling from the single gene, protein or cell level to large-scale arrays of proteomic, phosphoproteomic and interactomic data in order to uncover previously unidentified plant MAPK signaling pathways and to tackle with the challenging task of substrate identification. Techniques for MAPK sequence analysis and subcellular localization helping to identify their substrates and subcellular compartmentalization are also provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant MAP Kinases: Methods and Protocols represents a collection of useful plant MAPK protocols written by experts in the field for researchers and students.

Table of contents (19 chapters)

  • Transient Expression in Arabidopsis Leaf Mesophyll Protoplast System for Cell-Based Functional Analysis of MAPK Cascades Signaling

    Im, Jong Hee (et al.)

    Pages 3-12

  • Quantification of Stress-Induced Mitogen-Activated Protein Kinase Expressional Dynamic Using Reverse Transcription Quantitative Real-Time PCR

    Křenek, Pavel (et al.)

    Pages 13-25

  • Analysis of MAPK Activities Using MAPK-Specific Antibodies

    Willmann, Roland (et al.)

    Pages 27-37

  • Detection of Protein Phosphorylation and Charge Isoforms Using Vertical One-Dimensional Isoelectric Focusing Gels

    Anderson, Jeffrey C. (et al.)

    Pages 39-46

  • Affinity-Based SDS PAGE Identification of Phosphorylated Arabidopsis MAPKs and Substrates by Acrylamide Pendant Phos-Tag™

    Komis, George (et al.)

    Pages 47-63

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-4939-0922-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-4939-0921-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-4589-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Plant MAP Kinases
Book Subtitle
Methods and Protocols
Editors
  • George Komis
  • Jozef Šamaj
Series Title
Methods in Molecular Biology
Series Volume
1171
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
Distribution Rights
Distribution rights for India: Delhi Book Store, New Delhi, India
eBook ISBN
978-1-4939-0922-3
DOI
10.1007/978-1-4939-0922-3
Hardcover ISBN
978-1-4939-0921-6
Softcover ISBN
978-1-4939-4589-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 266
Number of Illustrations and Tables
16 b/w illustrations, 17 illustrations in colour
Topics