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  • © 2014

Transcription Factor Regulatory Networks

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1164)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
  2. Detecting Protein–Protein Interactions/Complex Components Using Mass Spectrometry Coupled Techniques

    • Zhibin Ning, Brett Hawley, Cheng-Kang Chiang, Deeptee Seebun, Daniel Figeys
    Pages 1-13
  3. Analysis of Transcription Factor Networks Using IVV Method

    • Hiroyuki Ohashi, Shigeo Fujimori, Naoya Hirai, Hiroshi Yanagawa, Etsuko Miyamoto-Sato
    Pages 15-22
  4. Chromatin Immunoprecipitation Protocol for Mammalian Cells

    • Makiko Komata, Yuki Katou, Hiroshi Tanaka, Ryuichiro Nakato, Katsuhiko Shirahige, Masashige Bando
    Pages 33-38
  5. Detecting Protein–DNA Interactions Using a Modified Yeast One-Hybrid System

    • Kazuhisa Ota, Shu-Ying Feng, Takashi Ito
    Pages 39-50
  6. RNA Sequencing: From Sample Preparation to Analysis

    • Sho Maekawa, Ayako Suzuki, Sumio Sugano, Yutaka Suzuki
    Pages 51-65
  7. Detecting Expressed Genes Using CAGE

    • Mitsuyoshi Murata, Hiromi Nishiyori-Sueki, Miki Kojima-Ishiyama, Piero Carninci, Yoshihide Hayashizaki, Masayoshi Itoh
    Pages 67-85
  8. Inference of TFRNs (2)

    • Hideo Matsuda
    Pages 97-107
  9. Modeling and Simulation Using CellDesigner

    • Yukiko Matsuoka, Akira Funahashi, Samik Ghosh, Hiroaki Kitano
    Pages 121-145
  10. Human Genome Network Platform: A Resource for TFRN Analysis

    • Norikazu Monma, Takashi Gojobori, Kazuho Ikeo
    Pages 147-162
  11. Identification of Transcription Factors Activated in Thymic Epithelial Cells During Embryonic Thymus Development

    • Taishin Akiyama, Junwen Qin, Daisuke Ohshima, Jun-ichiro Inoue
    Pages 163-170
  12. Analysis of TFRNs Associated with Steroid Hormone-Related Cancers

    • Kazuhiro Ikeda, Kuniko Horie-Inoue, Satoshi Inoue
    Pages 197-209
  13. Repositioning Monocyte TFRN into Fibroblasts

    • Takahiro Suzuki, Jay W. Shin, Harukazu Suzuki
    Pages 211-218
  14. Back Matter

    Pages 219-220

About this book

Transcription Factor Regulatory Methods details various techniques ranging from cutting-edge to general techniques use to study transcription factor regulatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Transcription Factor Regulatory Methods aids scientists in the further study into post-genomic or the personal genomic era.

Editors and Affiliations

  • Division of Interactome Medical Science, The University of Tokyo, The Institute of Medical Science, Minato-ku, Japan

    Etsuko Miyamoto-Sato, Hiroyuki Ohashi, Hirotaka Sasaki, Jun-ichi Nishikawa

  • Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University, Kohoku-ku, Yokohama, Japan

    Hiroshi Yanagawa

Bibliographic Information

  • Book Title: Transcription Factor Regulatory Networks

  • Book Subtitle: Methods and Protocols

  • Editors: Etsuko Miyamoto-Sato, Hiroyuki Ohashi, Hirotaka Sasaki, Jun-ichi Nishikawa, Hiroshi Yanagawa

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0805-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2014

  • Hardcover ISBN: 978-1-4939-0804-2Published: 14 June 2014

  • Softcover ISBN: 978-1-4939-5445-2Published: 17 September 2016

  • eBook ISBN: 978-1-4939-0805-9Published: 13 June 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 220

  • Number of Illustrations: 14 b/w illustrations, 53 illustrations in colour

  • Topics: Human Genetics, Gene Function

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access