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  • © 2014

Yeast Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1163)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-x
  2. Basic Investigations in Saccharomyces cerevisiae

    • Brendan P. G. Curran, Virginia Bugeja
    Pages 1-14
  3. Isolation of Yeast Nucleic Acids

    • Michael Biss, Michelle D. Hanna, Wei Xiao
    Pages 15-21
  4. Targeted Gene Deletion in Saccharomyces cerevisiae and Schizosaccharomyces pombe

    • Johannes H. Hegemann, Sven Boris Heick, Jennifer Pöhlmann, Marcus M. Langen, Ursula Fleig
    Pages 45-73
  5. Yeast Two-Hybrid Liquid Screening

    • Elisa Donnard, Erica M. Queiroz, J. Miguel Ortega, R. Daniel Gietz
    Pages 97-107
  6. Targeted Mutagenesis of a Specific Gene in Yeast

    • Likui Zhang, Alina Radziwon, Linda J. Reha-Krantz
    Pages 109-129
  7. Assays for Autophagy I: The Cvt Pathway and Nonselective Autophagy

    • Wei-Pang Huang, Takahiro Shintani, Zhiping Xie
    Pages 153-164
  8. Assays for Autophagy II: Mitochondrial Autophagy

    • Tomotake Kanki, Koji Okamoto
    Pages 165-173
  9. Yeast Survival and Growth Assays

    • Xin Xu, Amanda D. Lambrecht, Wei Xiao
    Pages 183-191
  10. Spontaneous Mutagenesis Assay

    • Susan Blackwell, Michelle D. Hanna, Wei Xiao
    Pages 193-199
  11. Replicative and Chronological Life-Span Assays

    • Spike D. L. Postnikoff, Troy A. A. Harkness
    Pages 223-227
  12. Detection of Protein Arginine Methylation in Saccharomyces cerevisiae

    • Christopher A. Jackson, Michael C. Yu
    Pages 229-247

About this book

Yeast Protocols, Third Edition presents up-to-date advances in research using yeasts as models. Chapters cover topics such as basic protocols in yeast culture and genomic manipulation, protocols that study certain organelles such as mitochondria and peroxisomes and their functions in autophagy and assays commonly used in yeast-based studies that can be adapted to other organisms. As the first sequenced living organism, budding yeast S. cerevisiae and other model yeasts have helped greatly in life science research. The easy switch between the haploid and diploid state makes yeast a paradigm of genetic manipulation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Yeast Protocols, Third Edition seeks to serve both professionals and novices with newly-developed protocols to study this essential model organism.

Editors and Affiliations

  • Capital Normal University College of Life Sciences, Beijing, China

    Wei Xiao

Bibliographic Information

  • Book Title: Yeast Protocols

  • Editors: Wei Xiao

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0799-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-0798-4Published: 20 May 2014

  • Softcover ISBN: 978-1-4939-4277-0Published: 17 September 2016

  • eBook ISBN: 978-1-4939-0799-1Published: 19 May 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: X, 313

  • Number of Illustrations: 30 b/w illustrations, 27 illustrations in colour

  • Topics: Microbiology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access