Methods in Molecular Biology

Quantitative Real-Time PCR

Methods and Protocols

Editors: Biassoni, Roberto, Raso, Alessandro (Eds.)

  • Includes cutting-edge methods and protocols in qPCR techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
see more benefits

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-4939-0733-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-4939-0732-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-4665-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation.

Reviews

From the book reviews:

“The book is a valuable collection of qPCR-related details that cover a wide range of PCR uses. Aimed mainly at researchers (wet lab biologists) with a solid PCR background, the book provides enough details for the novice too, who may find the numerous examples as well as the end notes in each chapter very useful.” (Irina Ioana Mohorianu, zbMATH, Vol. 1295, 2014)

Table of contents (17 chapters)

  • Twenty Years of qPCR: A Mature Technology?

    Raso, Alessandro (et al.)

    Pages 1-3

  • Minimum Information Necessary for Quantitative Real-Time PCR Experiments

    Johnson, Gemma (et al.)

    Pages 5-17

  • Selection of Reliable Reference Genes for RT-qPCR Analysis

    Hellemans, Jan (et al.)

    Pages 19-26

  • Introduction to Digital PCR

    Bizouarn, Francisco

    Pages 27-41

  • mRNA and microRNA Purity and Integrity: The Key to Success in Expression Profiling

    Kirchner, Benedikt (et al.)

    Pages 43-53

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-4939-0733-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-4939-0732-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-4665-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Quantitative Real-Time PCR
Book Subtitle
Methods and Protocols
Editors
  • Roberto Biassoni
  • Alessandro Raso
Series Title
Methods in Molecular Biology
Series Volume
1160
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-0733-5
DOI
10.1007/978-1-4939-0733-5
Hardcover ISBN
978-1-4939-0732-8
Softcover ISBN
978-1-4939-4665-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 231
Number of Illustrations and Tables
14 b/w illustrations, 66 illustrations in colour
Topics