Methods in Molecular Biology

Yeast Metabolic Engineering

Methods and Protocols

Editors: Mapelli, Valeria (Ed.)

  • Includes cutting-edge methods and protocols for the metabolic engineering of numerous strains of yeast
  • Provides step-by-step detail essential for reproducible results in the lab
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA (gross)
  • ISBN 978-1-4939-0563-8
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  • Included format: EPUB, PDF
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  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-4939-0562-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
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  • ISBN 978-1-4939-5286-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications. Beginning with an extensive section on molecular tools and technology for yeast engineering, this detailed volume is not limited to methods for Saccharomyces cerevisiae, but describes tools and protocols for engineering other yeasts of biotechnological interest, such as Pichia pastoris, Hansenula polymorpha and Zygosaccharomyces bailii. Tools and technologies for the investigation and determination of yeast metabolic features are described in detail as well as metabolic models and their application for yeast metabolic engineering, while a chapter describing patenting and regulations with a special glance at yeast biotechnology closes the volume. Written in the highly successful Methods in Molecular Biology series format, most chapters include an introduction to their respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Yeast Metabolic Engineering: Methods and Protocols aims to familiarize researchers with the current state of these vital and increasingly useful technologies.

Table of contents (19 chapters)

  • An Overview on Selection Marker Genes for Transformation of Saccharomyces cerevisiae

    Siewers, Verena

    Pages 3-15

  • Natural and Modified Promoters for Tailored Metabolic Engineering of the Yeast Saccharomyces cerevisiae

    Hubmann, Georg (et al.)

    Pages 17-42

  • Tools for Genetic Engineering of the Yeast Hansenula polymorpha

    Saraya, Ruchi (et al.)

    Pages 43-62

  • Molecular Tools and Protocols for Engineering the Acid-Tolerant Yeast Zygosaccharomyces bailii as a Potential Cell Factory

    Branduardi, Paola (et al.)

    Pages 63-85

  • Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris

    Felber, Michael (et al.)

    Pages 87-111

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-4939-0563-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-4939-0562-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-5286-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Yeast Metabolic Engineering
Book Subtitle
Methods and Protocols
Editors
  • Valeria Mapelli
Series Title
Methods in Molecular Biology
Series Volume
1152
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-4939-0563-8
DOI
10.1007/978-1-4939-0563-8
Hardcover ISBN
978-1-4939-0562-1
Softcover ISBN
978-1-4939-5286-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 316
Number of Illustrations and Tables
26 b/w illustrations, 18 illustrations in colour
Topics