Methods in Molecular Biology

Herpes Simplex Virus

Methods and Protocols

Editors: Diefenbach, Russell J., Fraefel, Cornel (Eds.)

  • Includes cutting-edge methods and protocols for the study of the complex interactions between herpes viruses and the host
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-4939-0428-0
  • Digitally watermarked, DRM-free
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  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-4939-0427-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-5407-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Herpes Simplex Virus: Methods and Protocols provides a wide collection of protocols employed in various levels of herpes virus research, including basic protocols on growing viruses in cell culture and cloning, manipulating and preparing viral DNA. Other chapters describe approaches to design and apply HSV-1 vectors for vaccination, cancer and gene therapy or to study specific aspects of HSV-1 biology such as latency, intracellular transport and protein-protein interaction. Procedures for structural analyses, microscopy, proteomics and testing of antivirals are included as well. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Herpes Simplex Virus: Methods and Protocols will aid new researchers in the field of herpes virology as well as those experienced investigators wishing to embark on new techniques.

Table of contents (21 chapters)

  • HSV-1 Biology and Life Cycle

    Everett, Roger D.

    Pages 1-17

  • Herpes Simplex Virus Growth, Preparation, and Assay

    Marconi, Peggy (et al.)

    Pages 19-29

  • Isolation of Herpes Simplex Virus Nucleocapsid DNA

    Szpara, Moriah

    Pages 31-41

  • Construction and Characterization of Bacterial Artificial Chromosomes (BACs) Containing Herpes Simplex Virus Full-Length Genomes

    Nagel, Claus-Henning (et al.)

    Pages 43-62

  • Engineering HSV-1 Vectors for Gene Therapy

    Goins, William F. (et al.)

    Pages 63-79

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-4939-0428-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-4939-0427-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-5407-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Herpes Simplex Virus
Book Subtitle
Methods and Protocols
Editors
  • Russell J. Diefenbach
  • Cornel Fraefel
Series Title
Methods in Molecular Biology
Series Volume
1144
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-0428-0
DOI
10.1007/978-1-4939-0428-0
Hardcover ISBN
978-1-4939-0427-3
Softcover ISBN
978-1-4939-5407-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 331
Number of Illustrations and Tables
17 b/w illustrations, 28 illustrations in colour
Topics