Methods in Molecular Biology

Dengue

Methods and Protocols

Editors: Padmanabhan, Radhakrishnan, Vasudevan, Subhash G. (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-4939-0348-1
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  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-4939-0347-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
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  • ISBN 978-1-4939-4371-5
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  • Usually dispatched within 3 to 5 business days.
About this book

Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact millions of lives and cause tens of thousands of mortalities each year. Dengue is a global public health emergency especially since there is no preventative vaccine or antiviral treatment for dengue disease. Dengue: Methods and Protocols offers the increasing number of dengue researchers a one-stop protocol book with techniques compiled from the leading laboratories working on dengue. Chapters cover topics such as dengue virus isolation from clinical samples, quantification of human antibodies against the virus, assays to quantify the virus particles, the widely used mouse model to study dengue pathogenesis, vaccine and antiviral efficacies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Dengue: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies on dengue research.

Table of contents (25 chapters)

  • Dengue Virus Growth, Purification, and Fluorescent Labeling

    Zhang, Summer (et al.)

    Pages 3-14

  • Isolation and Titration of Dengue Viruses by the Mosquito Inoculation Technique

    Choy, Milly M. (et al.)

    Pages 15-25

  • Measuring Antibody Neutralization of Dengue Virus (DENV) Using a Flow Cytometry-Based Technique

    Alwis, Ruklanthi (et al.)

    Pages 27-39

  • Dengue Virus Purification and Sample Preparation for Cryo-Electron Microscopy

    Tan, Joanne L. (et al.)

    Pages 41-52

  • Development of a Multiplex Bead-Based Assay to Monitor Dengue Virus Seroconversion

    Ng, Kaiting (et al.)

    Pages 53-74

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-4939-0348-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-4939-0347-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-4371-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Dengue
Book Subtitle
Methods and Protocols
Editors
  • Radhakrishnan Padmanabhan
  • Subhash G. Vasudevan
Series Title
Methods in Molecular Biology
Series Volume
1138
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
Distribution Rights
Distribution rights for India: Researchco Book Centre, New Delhi, India
eBook ISBN
978-1-4939-0348-1
DOI
10.1007/978-1-4939-0348-1
Hardcover ISBN
978-1-4939-0347-4
Softcover ISBN
978-1-4939-4371-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 403
Number of Illustrations and Tables
33 b/w illustrations, 49 illustrations in colour
Topics