Models for Assessing Drug Absorption and Metabolism

1. Front Matter

   Pages i-xxii

   PDF

2. General Principles in the Characterization and Use of Model Systems for Biopharmaceutical Studies

   • Ronald T. Borchardt, Philip L. Smith, Glynn Wilson

   Pages 1-11

3. Methods for Evaluating Intestinal Permeability and Metabolism in Vitro

   • Philip L. Smith

   Pages 13-34

4. Cultured Intestinal Epithelial Cell Models

   • Ismael J. Hidalgo

   Pages 35-50

5. Intestinal Rings and Isolated Intestinal Mucosal Cells

   • Joseph A. Fix

   Pages 51-66

6. Models of Drug Absorption in Situ and in Conscious Animals

   • Robin Griffiths, Ann Lewis, Phillip Jeffrey

   Pages 67-84

7. Model Systems for Intestinal Lymphatic Transport Studies

   • Christopher J. H. Porter, William N. Charman

   Pages 85-102

8. Buccal Tissues and Cell Culture

   • Elizabeth Quadros, James P. Cassidy, Harry Leipold

   Pages 103-113

9. Isolated Hepatocytes

   • M. Vore, Y. Liu, T. C. Ganguly

   Pages 115-120

10. Cultured Rat Hepatocytes

    • Edward L. LeCluyse, Peter L. Bullock, Andrew Parkinson, Jerome H. Hochman

    Pages 121-159

11. Isolated Perfused Liver

    • Kim L. R. Brouwer, Ronald G. Thurman

    Pages 161-192

12. Isolated Renal Brush Border and Basolateral Membrane Vesicles and Cultured Renal Cells

    • Marcelo M. Gutierrez, Claire M. Brett, Kathleen M. Giacomini

    Pages 193-210

13. Use of an Isolated Perfused Kidney to Assess Renal Clearance of Drugs

    • Noriko Okudaira, Yuichi Sugiyama

    Pages 211-238

14. Brain Microvessel Endothelial Cell Culture Systems

    • Kenneth L. Audus, Lawrence Ng, Wen Wang, Ronald T. Borchardt

    Pages 239-258

15. Methods to Study Drug Transport in Isolated Choroid Plexus Tissue and Cultured Cells

    • Carla B. Washington, Kathleen M. Giacomini, Claire M. Brett

    Pages 259-283

16. Brain Perfusion Systems for Studies of Drug Uptake and Metabolism in the Central Nervous System

    • Quentin R. Smith

    Pages 285-307

17. In Vitro Nasal Models

    • Patricia M. Reardon

    Pages 309-323

18. Models for Investigation of Peptide and Protein Transport across Cultured Mammalian Respiratory Epithelial Barriers

    • Kwang-Jin Kim, Edward D. Crandall

    Pages 325-346

19. Drug Transport across Xenopus Alveolar Epithelium in Vitro

    • Doris Wall, Doreen Pierdomenico

    Pages 347-359

20. In Situ and in Vivo Methods for Pulmonary Delivery

    • Mohammed Eljamal, Sudha Nagarajan, John S. Patton

    Pages 361-374

Pharmaceutical scientists in industry and academia will appreciate this single reference for its detailed experimental procedures for conducting biopharmaceutical studies. This well-illustrated guide allows them to establish, validate, and implement commonly used in situ and in vitro model systems. Chapters provide ready access to these methodologies for studies of the intestinal, buccal, nasal and respiratory, vaginal, ocular, and dermal epithelium as well as the endothelial and elimination barriers.

• absorption
• drug
• drugs
• kidney
• metabolism
• pharmaceutical
• research
• transport

• The University of Kansas, Lawrence, USA

  Ronald T. Borchardt

• SmithKline Beecham Pharmaceuticals, King of Prussia, USA

  Philip L. Smith

• Tacora Corporation, Seattle, USA

  Glynn Wilson

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