Biological Separations

Methods of Protein Separation

Editors: Catsimpoolas, Nicholas (Ed.)

Buy this book

eBook N/A
  • ISBN 978-1-4757-1257-5
  • digitally watermarked, no DRM
  • included format: PDF
  • eBooks can be used on all Reading Devices
About this book

This open-end treatise on methods concerning protein separation had its beginning in an American Chemical Society symposium entitled "Con­ temporary Protein Separation Methods" which was held in Atlantic City, New Jersey in September 1974. The purpose of the symposium-and subse­ quently of the present work-was to review the available modern techniques and underlying principles for achieving one of the very important tasks of experimental biology, namely the separation and characterization of proteins present in complex biological mixtures. Physicochemical characterization was covered only as related to the parent method of fractionation and there­ fore involved mostly mass transport processes. Additionally, the presentation of methods for gaini. ng insight into complex interacting protein profiles was considered of paramount importance in the interpretation of separation patterns. Finally, specific categories of proteins (e. g. , chemically modified, deriving from a specific tissue, conjugated to different moieties, etc. ) require meticulous trial and selection andjor modification of existing methodology to carry out the desired separation. In such cases, the gained experience provides valuable guidelines for further experimentation. Although powerful techniques exist today for the separation and related physicochemical characterization of proteins, many biological fractionation problems require further innovations. It is hoped that the description in the present treatise of some of the available separation tools and their limitations will provide the necessary integrated background for new developments in this area.

Table of contents (1 chapter)

  • Affinity Chromatography, Principles and Applications

    Indu Parikh, Pedro Cuatrecasas

    Pages 255-276

    Buy Chapter price n/a
Free Preview

Buy this book

eBook N/A
  • ISBN 978-1-4757-1257-5
  • digitally watermarked, no DRM
  • included format: PDF
  • eBooks can be used on all Reading Devices

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Methods of Protein Separation
Series Title
Biological Separations
Copyright
1975
Publisher
Springer US
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4757-1257-5
DOI
10.1007/978-1-4757-1257-5
Edition Number
1
Topics