Biological Separations

Methods of Protein Separation

Editors: Catsimpoolas, Nicholas (Ed.)

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About this book

This open-end treatise on methods concerning protein separation had its beginning in an American Chemical Society symposium entitled "Con­ temporary Protein Separation Methods" which was held in Atlantic City, New Jersey in September 1974. The purpose of the symposium-and subse­ quently of the present work-was to review the available modern techniques and underlying principles for achieving one of the very important tasks of experimental biology, namely the separation and characterization of proteins present in complex biological mixtures. Physicochemical characterization was covered only as related to the parent method of fractionation and there­ fore involved mostly mass transport processes. Additionally, the presentation of methods for gaini. ng insight into complex interacting protein profiles was considered of paramount importance in the interpretation of separation patterns. Finally, specific categories of proteins (e. g. , chemically modified, deriving from a specific tissue, conjugated to different moieties, etc. ) require meticulous trial and selection andjor modification of existing methodology to carry out the desired separation. In such cases, the gained experience provides valuable guidelines for further experimentation. Although powerful techniques exist today for the separation and related physicochemical characterization of proteins, many biological fractionation problems require further innovations. It is hoped that the description in the present treatise of some of the available separation tools and their limitations will provide the necessary integrated background for new developments in this area.

Table of contents (9 chapters)

  • Sedimentation and Gel-Permeation Chromatography of Associating-Dissociating Macromolecules

    Cann, John R.

    Pages 1-25

  • Trans Electrophoresis

    Catsimpoolas, Nicholas

    Pages 27-67

  • Immunodiffusion

    Crowle, Alfred J.

    Pages 69-92

  • Isoelectric Focusing in Polyacrylamide Gel

    Drysdale, James W.

    Pages 93-126

  • Purification of Chemically Modified Proteins

    Feeney, Robert E. (et al.)

    Pages 127-160

Buy this book

eBook $69.99
price for USA (gross)
  • ISBN 978-1-4757-1257-5
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
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Bibliographic Information

Bibliographic Information
Book Title
Methods of Protein Separation
Editors
  • Nicholas Catsimpoolas
Series Title
Biological Separations
Copyright
1975
Publisher
Springer US
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4757-1257-5
DOI
10.1007/978-1-4757-1257-5
Edition Number
1
Number of Pages
XIII, 281
Number of Illustrations and Tables
125 b/w illustrations
Topics