Biological Magnetic Resonance

Distance Measurements in Biological Systems by EPR

Editors: Berliner, Lawrence J., Eaton, Sandra S., Eaton, Gareth R. (Eds.)

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About this book

Distance measurements in biological systems by EPR The foundation for understanding function and dynamics of biological systems is knowledge of their structure. Many experimental methodologies are used for determination of structure, each with special utility. Volumes in this series on Biological Magnetic Resonance emphasize the methods that involve magnetic resonance. This volume seeks to provide a critical evaluation of EPR methods for determining the distances between two unpaired electrons. The editors invited the authors to make this a very practical book, with specific numerical examples of how experimental data is worked up to produce a distance estimate, and realistic assessments of uncertainties and of the range of applicability, along with examples of the power of the technique to answer biological problems. The first chapter is an overview, by two of the editors, of EPR methods to determine distances, with a focus on the range of applicability. The next chapter, also by the Batons, reviews what is known about electron spin relaxation times that are needed in estimating distances between spins or in selecting appropriate temperatures for particular experiments. Albert Beth and Eric Hustedt describe the information about spin-spin interaction that one can obtain by simulating CW EPR line shapes of nitroxyl radicals. The information in fluid solution CW EPR spectra of dual-spin labeled proteins is illustrated by Hassane Mchaourab and Eduardo Perozo.

About the authors

Dr. Lawrence J. Berliner is currently Professor and Chair of the Department of Chemistry and Biochemistry at the University of Denver after retiring from Ohio State University, where he spent a 32-year career in the area of biological magnetic resonance (EPR and NMR). He is the Series Editor for Biological Magnetic Resonance, which he launched in 1979.

Table of contents (13 chapters)

  • Distance Measurements by CW and Pulsed EPR

    Eaton, Sandra S. (et al.)

    Pages 1-27

  • Relaxation Times of Organic Radicals and Transition Metal Ions

    Eaton, Sandra S. (et al.)

    Pages 29-154

  • Structural Information from CW-EPR Spectra of Dipolar Coupled Nitroxide Spin Labels

    Hustedt, Eric J. (et al.)

    Pages 155-184

  • Determination of Protein Folds and Conformational Dynamics Using Spin-Labeling EPR Spectroscopy

    Mchaourab, Hassane S. (et al.)

    Pages 185-247

  • EPR Spectroscopic Ruler: the Method and its Applications

    Xiao, Wenzhong (et al.)

    Pages 249-276

Buy this book

eBook $289.00
price for USA (gross)
  • ISBN 978-0-306-47109-4
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $369.00
price for USA
  • ISBN 978-1-4757-0575-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Distance Measurements in Biological Systems by EPR
Editors
  • Lawrence J. Berliner
  • Sandra S. Eaton
  • Gareth R. Eaton
Series Title
Biological Magnetic Resonance
Series Volume
19
Copyright
2000
Publisher
Springer US
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-0-306-47109-4
DOI
10.1007/b111467
Softcover ISBN
978-1-4757-0575-1
Series ISSN
0192-6020
Edition Number
1
Number of Pages
XVIII, 614
Topics