Biological Separations

Methods of Cell Separation

Editors: Catsimpoolas, Nicholas (Ed.)

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About this book

Presently, the need for methods involving separation, identification, and characterization of different kinds of cells is amply realized among immu­ nologists, hematologists, cell biologists, clinical pathologists, and cancer researchers. Unless cells exhibiting different functions and stages of differ­ entiation are separated from one another, it will be exceedingly difficult to study some of the molecular mechanisms involved in cell recognition, specialization, interactions, cytotoxicity, and transformation. Clinical diag­ nosis of diseased states and use of isolated cells for therapeutic (e. g. , immunotherapy) or survival (e. g. , transfusion) purposes are some of the pressing areas where immediate practical benefits can be obtained by applying cell separation techniques. However. the development of such useful methods is still in its infancy. A number of good techniques exist based either on the physical or biological properties of the cells, and these have produced some valuable results. Still others are to be discovered. Therefore, the purpose of this open-end treatise is to acquaint the reader with some of the basic principles, instrumentation, and procedures pres­ ently in practice at various laboratories around the world and to present some typical applications of each technique to particular biological prob­ lems. To this end, I was fortunate to obtain the contribution of certain leading scientists in the field of cell separation, people who in their pioneer­ ing work have struggled with the particular problems involved in separating living cells and in some way have won.

Table of contents (8 chapters)

  • Preparative Density Gradient Electrophoresis and Velocity Sedimentation at Unit Gravity of Mammalian Cells

    Catsimpoolas, Nicholas (et al.)

    Pages 1-24

  • Isopycnic Separation of Cells and Cell Organelles by Centrifugation in Modified Colloidal Silica Gradients

    Pertoft, Håkan (et al.)

    Pages 25-65

  • Dielectrophoresis: Applications to the Characterization and Separation of Cells

    Pohl, Herbert A.

    Pages 67-169

  • Separation of Viable Cells by Velocity Sedimentation in an Isokinetic Gradient of Ficoll in Tissue Culture Medium

    Pretlow, Thomas G. (et al.)

    Pages 171-191

  • Fractionation and Manipulation of Cells with Chemically Modified Fibers and Surfaces

    Rutishauser, Urs S. (et al.)

    Pages 193-228

Buy this book

eBook $69.99
price for USA (gross)
  • ISBN 978-1-4684-0820-1
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
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Bibliographic Information

Bibliographic Information
Book Title
Methods of Cell Separation
Editors
  • Nicholas Catsimpoolas
Series Title
Biological Separations
Copyright
1977
Publisher
Springer US
Copyright Holder
Springer-Verlag US
eBook ISBN
978-1-4684-0820-1
DOI
10.1007/978-1-4684-0820-1
Edition Number
1
Topics