Single-Channel Recording

Editors: Sakmann, Bert (Ed.)

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About this book

Single-channel recording has become a widely used tool for the study of ion permeation mechanisms in biological membranes. Whereas the technique might have been considered an "art" after its introduction in 1976, it developed into a relatively simple method after it became possible to obtain high-resistance (several gigaohm) membrane-pipette seals. In the summer of 1982, a course on the technique was held at the Ettore Majorana Center for Scientific Culture in Erice, Sicily. It brought together people from most of the laboratories involved in patch clamping at that time. During the course, it became apparent that the technique had reached a state of maturity. Repeatedly, the opinion was expressed that a detailed description of all the aspects of the technique including representative examples of results should be available. We therefore asked the course instructors, as well as several other colleagues, to provide chapters on selected topics in order to produce this volume. The different variants of patch clamping were described quite extensively in an article by Hamill, Marty, Neher, Sakmann, and Sigworth (Pflugers Archiv 391:85) in 1981. Rather than repeating this survey in an introductory chapter, we chose to reprint that article in the Appendix of this volume (by permission of Springer-Verlag). The methods section will, therefore, go straight into detailed aspects of the technol­ ogy.

Table of contents (25 chapters)

  • Electronic Design of the Patch Clamp

    Sigworth, F. J.

    Pages 3-35

  • Geometric Parameters of Pipettes and Membrane Patches

    Sakmann, B. (et al.)

    Pages 37-51

  • Science and Technology of Patch-Recording Electrodes

    Corey, D. P. (et al.)

    Pages 53-68

  • Enzymatic Dispersion of Heart and Other Tissues

    Trube, G.

    Pages 69-76

  • A Primer in Cell Culture for Patchologists

    Spector, Ilan

    Pages 77-90

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-4615-7858-1
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $139.99
price for USA
  • ISBN 978-1-4615-7860-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Single-Channel Recording
Editors
  • Bert Sakmann
Copyright
1983
Publisher
Springer US
Copyright Holder
Plenum Press, New York
eBook ISBN
978-1-4615-7858-1
DOI
10.1007/978-1-4615-7858-1
Softcover ISBN
978-1-4615-7860-4
Edition Number
1
Number of Pages
XXII, 504
Number of Illustrations and Tables
136 b/w illustrations
Topics