Methods in Molecular Biology

Electroporation Protocols

Preclinical and Clinical Gene Medicine

Editors: Li, S., Cutrera, J., Heller, R., Teissie, J. (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-4614-9632-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-4614-9631-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-5492-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Electroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture and discovery and synthesis of siRNA. Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues and for internal organs. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition aims to provide not only comprehensive coverage of the basic theory and practical application of electroporation siRNA therapy, gene therapy, and vaccine, but also elaborates on the most current views from the experts in this field, serving as an invaluable resource for investigators both in and outside of this field.

Table of contents (30 chapters)

  • Electroporation-Based Gene Therapy: Recent Evolution in the Mechanism Description and Technology Developments

    Mir, Lluis M.

    Pages 3-23

  • Electropermeabilization of the Cell Membrane

    Teissie, Justin

    Pages 25-46

  • The Impact of Non-electrical Factors on Electrical Gene Transfer

    Hu, Jiemiao (et al.)

    Pages 47-54

  • Electroporation Formulation for Cell Therapy

    Hu, Jiemiao (et al.)

    Pages 55-60

  • Short-Fragment DNA-Mediated In Vivo DNA Electroporation Delivery

    Peng, Jinliang (et al.)

    Pages 61-67

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-4614-9632-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-4614-9631-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-5492-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Electroporation Protocols
Book Subtitle
Preclinical and Clinical Gene Medicine
Editors
  • Shulin Li
  • Jeffry Cutrera
  • Richard Heller
  • Justin Teissie
Series Title
Methods in Molecular Biology
Series Volume
1121
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4614-9632-8
DOI
10.1007/978-1-4614-9632-8
Hardcover ISBN
978-1-4614-9631-1
Softcover ISBN
978-1-4939-5492-6
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XIV, 349
Number of Illustrations and Tables
20 b/w illustrations, 49 illustrations in colour
Topics