Skip to main content
SpringerLink
Log in

Methods in Cellular Imaging

  • Book
  • © 2001

Overview

Editors:
  1. Ammasi Periasamy

    1. W. M. Keck Center for Cellular Imaging, University of Virginia, Charlottesville, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Well illustrated book explains basic concepts and imaging procedures
  • Authors descrube approaches to selecting epiflourescence microscopy, detectors, and image acquisition and processing software for different biological adaptations
  • Written for graduate students and scientists

Part of the book series: Methods in Physiology (METHPHYS)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 119.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (24 chapters)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Basics of Fluorescence, Fluorophores, Microscopy, and Detectors

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Basics of Fluorescence

      • Keith Berland
      Pages 5-19

    3. Fluorophores and Their Labeling Procedures for Monitoring Various Biological Signals

      • Ian S. Harper
      Pages 20-39

    4. Detectors for Fluorescence Microscopy

      • Kenneth R. Spring
      Pages 40-52

    5. Basics of a Light Microscopy Imaging System and Its Application in Biology

      • Lance Davidson, Raymond Keller
      Pages 53-65

    6. Laser Scanning Confocal Microscopy Applied to Living Cells and Tissues

      • John J. Lemasters, Ting Qian, Donna R. Trollinger, Barbara J. Muller-Borer, Steven P. Elmore, Wayne E. Cascio
      Pages 66-87

    7. Functional Imaging of Mitochondria Within Cells

      • Michael R. Duchen, Jake Jacobson, Julie Keelan, Mart H. Mojet, Olga Vergun
      Pages 88-111

    8. Diffusion Measurements by Fluorescence Recovery After Photobleaching

      • Alan S. Verkman, Lakshmanan Vetrivel, Peter Haggie
      Pages 112-127

    9. Processing Microscope-Acquired Images for Use in Multimedia, Print, and the World Wide Web

      • Harold L. Noakes Jr., Ammasi Periasamy
      Pages 128-142

  3. Multiphoton Excitation Fluorescence Microscopy

    1. Front Matter

      Pages 143-143
      Download chapter PDF

    2. Basic Principles of Multiphoton Excitation Microscopy

      • Peter T. C. So, Ki H. Kim, Christof Buehler, Barry R. Masters, Lily Hsu, Chen-Yuan Dong
      Pages 147-161

    3. Building a Two-Photon Microscope Using a Laser Scanning Confocal Architecture

      • Alberto Diaspro
      Pages 162-179

    4. Two-Photon Microscopy in Highly Scattering Tissue

      • Vincent P. Wallace, Andrew K. Dunn, Mariah L. Coleno, Bruce J. Tromberg
      Pages 180-199

    5. Multiphoton Laser Scanning Microscopy and Dynamic Imaging in Embryos

      • Mary E. Dickinson, Scott E. Fraser
      Pages 200-215

    6. In vivo Diffusion Measurements Using Multiphoton Excitation Fluorescence Photobleaching Recovery and Fluorescence Correlation Spectroscopy

      • Warren R. Zipfel, Watt W. Webb
      Pages 216-235

    7. Cellular Response to Laser Radiation in Fluorescence Microscopes

      • Karsten König
      Pages 236-251

  4. Fluorescence Resonance Energy Transfer and Lifetime Imaging Microscopy

    1. Front Matter

      Pages 253-256
      Download chapter PDF

    2. Measurement of Fluorescence Resonance Energy Transfer in the Optical Microscope

      • Brian Herman, Gerald Gordon, Nupam Mahajan, Victoria Centonze
      Pages 257-272

    3. Frequency-Domain Fluorescence Lifetime Imaging Microscopy: A Window on the Biochemical Landscape of the Cell

      • Peter J. Verveer, Anthony Squire, Philippe I. H. Bastiaens
      Pages 273-294
Back to top

Keywords

About this book

Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton excitation, fluorescence resonance energy transfer (FRET), lifetime imaging (FLIM), spectral imaging, fluorescence recovery after photobleaching (FRAP), optical tweezers, total internal reflection, high spatial resolution atomic force microscopy (AFM), and bioluminescence imaging for gene expression. The usage of these techniques in various biological applications, including calcium, pH, membrane potential, mitochondrial signaling, protein-protein interactions under various physiological conditions, and deep tissue imaging, is clearly presented. The authors describe the approaches to selecting epifluorescence microscopy, the detectors, and the image acquisition and processing software for different biological applications. Step-by-step directions on preparing different digital formats for light microscopy images on websites are also provided.

Editors and Affiliations

  • W. M. Keck Center for Cellular Imaging, University of Virginia, Charlottesville, USA

    Ammasi Periasamy

Bibliographic Information

  • Book Title: Methods in Cellular Imaging

  • Editors: Ammasi Periasamy

  • Series Title: Methods in Physiology

  • DOI: https://doi.org/10.1007/978-1-4614-7513-2

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: American Physiological Society 2001

  • eBook ISBN: 978-1-4614-7513-2Published: 27 May 2013

  • Series ISSN: 2628-7471

  • Series E-ISSN: 2628-748X

  • Edition Number: 1

  • Number of Pages: 448

  • Additional Information: Originally Published by Oxford University Press 2001

  • Topics: Cell Physiology

Publish with us

Policies and ethics

Back to top

Search

Navigation