Methods in Physiology

Methods in Cellular Imaging

Editors: Periasamy, Ammasi (Ed.)

  • Well illustrated book explains basic concepts and imaging procedures
  • Authors descrube approaches to selecting epiflourescence microscopy, detectors, and image acquisition and processing software for different biological adaptations
  • Written for graduate students and scientists
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eBook $119.00
price for USA (gross)
  • ISBN 978-1-4614-7513-2
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About this book

Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton excitation, fluorescence resonance energy transfer (FRET), lifetime imaging (FLIM), spectral imaging, fluorescence recovery after photobleaching (FRAP), optical tweezers, total internal reflection, high spatial resolution atomic force microscopy (AFM), and bioluminescence imaging for gene expression. The usage of these techniques in various biological applications, including calcium, pH, membrane potential, mitochondrial signaling, protein-protein interactions under various physiological conditions, and deep tissue imaging, is clearly presented. The authors describe the approaches to selecting epifluorescence microscopy, the detectors, and the image acquisition and processing software for different biological applications. Step-by-step directions on preparing different digital formats for light microscopy images on websites are also provided.

Table of contents (24 chapters)

  • Basics of Fluorescence

    Berland, Keith

    Pages 5-19

  • Fluorophores and Their Labeling Procedures for Monitoring Various Biological Signals

    Harper, Ian S.

    Pages 20-39

  • Detectors for Fluorescence Microscopy

    Spring, Kenneth R.

    Pages 40-52

  • Basics of a Light Microscopy Imaging System and Its Application in Biology

    Davidson, Lance (et al.)

    Pages 53-65

  • Laser Scanning Confocal Microscopy Applied to Living Cells and Tissues

    Lemasters, John J. (et al.)

    Pages 66-87

Buy this book

eBook $119.00
price for USA (gross)
  • ISBN 978-1-4614-7513-2
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
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Bibliographic Information

Bibliographic Information
Book Title
Methods in Cellular Imaging
Editors
  • Ammasi Periasamy
Series Title
Methods in Physiology
Copyright
2001
Publisher
Springer-Verlag New York
Copyright Holder
American Physiological Society
eBook ISBN
978-1-4614-7513-2
DOI
10.1007/978-1-4614-7513-2
Edition Number
1
Number of Pages
448
Additional Information
Originally Published by Oxford University Press 2001
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