Methods in Molecular Biology

The Low Molecular Weight Proteome

Methods and Protocols

Editors: Bäckvall, Helena, Lehtiö, Janne (Eds.)

  • Presents current up-to-date methodologies
  • Features tips on trouble shooting and avoiding known pitfalls
  • Contains key notes and implementation advice from the experts
see more benefits

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-4614-7209-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-4614-7167-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-5567-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Small proteins with molecular weights of <25 kDa are involved in major biological processes such as ribosome formation, stress  adaption and cell cycle control. The study of the low-molecular-weight proteome has identified many central regulators of biology such as cytokines, chemokines, peptide hormones and proteolytic fragments of larger proteins.  Due to the unique features of these proteins, the technical challenges are different  from those in ”common” proteomics. In The Low Molecular Weight Proteome: Methods and Protocols expert researchers from the field provide protocols for analysis of low molecular weight proteins and peptides, protocols for such methods applied in clinical research and an up-to-date review of quantitative protein profiling by labeling.  These include methods suitable for both peptide and protein analysis with focus on methods and application that can be used for small protein analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, The Low Molecular Weight Proteome: Methods and Protocolsis a useful resource for experienced proteomics practitioners as well as an aid to newcomers who wish to become acquainted with the theory and practice of a wide array of methods in analyzing small proteins or peptides.

Table of contents (14 chapters)

  • Narrow-Range Peptide Isoelectric Focusing as Peptide Prefractionation Method Prior to Tandem Mass Spectrometry Analysis

    Pernemalm, Maria

    Pages 3-11

  • Analysis of Peptides by Denaturing Ultrafiltration and LC-MALDI-TOF-MS

    An, Yanming (et al.)

    Pages 13-19

  • Stable Isotope Labeling Methods in Protein Profiling

    Lengqvist, Johan (et al.)

    Pages 21-51

  • Development of MRM-Based Assays for the Absolute Quantitation of Plasma Proteins

    Kuzyk, Michael A. (et al.)

    Pages 53-82

  • Mass Spectrometric Profiling of Low-Molecular-Weight Proteins

    Rainer, Matthias (et al.)

    Pages 83-95

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-4614-7209-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-4614-7167-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-5567-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
The Low Molecular Weight Proteome
Book Subtitle
Methods and Protocols
Editors
  • Helena Bäckvall
  • Janne Lehtiö
Series Title
Methods in Molecular Biology
Series Volume
1023
Copyright
2013
Publisher
Springer-Verlag New York
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4614-7209-4
DOI
10.1007/978-1-4614-7209-4
Hardcover ISBN
978-1-4614-7167-7
Softcover ISBN
978-1-4939-5567-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 223
Number of Illustrations and Tables
24 b/w illustrations, 26 illustrations in colour
Topics