Subcellular Biochemistry

Physicochemical Methods in the Study of Biomembranes

Editors: Hilderson, Herwig J., Ralston, Gregory B. (Eds.)

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About this book

In mammalian cells many physiological processes rely on the dynamics of the organization of lipids and proteins in biological membranes. The topics in this volume deal with physicochemical methods in the study of biomembranes. Some of them have a long and respectable history in the study of soluble proteins and have only recently been applied to the study of membranes. Some have tradi­ tionally been applied to studies of model systems of lipids of well-defined com­ position, as well as to intact membranes. Other methods, by their very nature, apply to organized bilayers comprised of both protein and lipid. Van Meer and van Genderen provide us with an introduction to the field (Chapter I). From their personal perspective regarding the distribution, trans­ port, and sorting of membrane lipids, they formulate a number of biologically relevant questions and show that the physicochemical methods described in this book may contribute in great measure to solving these issues. The methods of analytical ultracentrifugation have served faithfully for 60 years in the study of water-soluble proteins. The use of detergent extraction of membrane proteins, and the manipulation of density with H20/D20 mixtures, has extended this technique to the study of proteins, and in particular their interactions, from biological membranes. As described by Morris and Ralston in Chapter 2, this technique can be used to determine a number of important properties of proteins.

Reviews

`Chapters are well written and informative and have extensive lists of references..the book is, in general, well produced.'
Journal of Magnetic Resonance

Table of contents (11 chapters)

  • Intracellular Lipid Distribution, Transport, and Sorting

    Meer, Gerrit (et al.)

    Pages 1-24

  • Biophysical Characterization of Membrane and Cytoskeletal Proteins by Sedimentation Analysis

    Morris, Michael B. (et al.)

    Pages 25-82

  • Monomolecular Layers in the Study of Biomembranes

    Demel, Rudy A.

    Pages 83-120

  • Differential Scanning and Dynamic Calorimetric Studies of Cooperative Phase Transitions in Phospholipid Bilayer Membranes

    Ye, Qiang (et al.)

    Pages 121-160

  • Ektacytometry of Red Cells

    Johnson, Robert M.

    Pages 161-203

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-4615-1863-1
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $119.00
price for USA
  • ISBN 978-1-4613-5757-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Physicochemical Methods in the Study of Biomembranes
Editors
  • Herwig J. Hilderson
  • Gregory B. Ralston
Series Title
Subcellular Biochemistry
Series Volume
23
Copyright
1994
Publisher
Springer US
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4615-1863-1
DOI
10.1007/978-1-4615-1863-1
Softcover ISBN
978-1-4613-5757-5
Series ISSN
0306-0225
Edition Number
1
Number of Pages
XXI, 509
Topics