Biological Separations

Biological and Biomedical Applications of Isoelectric Focusing

Editors: Catsimpoolas, Nicholas (Ed.)

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About this book

It has now been over a decade since isoelectric focusing became estab­ lished as a valuable addition to our arsenal of physical methods for separa­ tion of proteins and other amphoteric substances. The high resolving power of the technique and its unique ability to separate as well as concentrate have been amply demonstrated in several international symposia. The past few years have led to the wide acceptance of standardized methodologies for analytical and preparative purposes. These procedures afford rapid, reproducible separations that have led to a dramatic increase in our under­ standing of many areas of biological and biomedical research. Conse­ quently, we considered it desirable to review some of the important appli­ cations of the isoelectric focusing technique. To accomplish this, we have solicited the participation of noted authorities to highlight major progress in their fields of expertise. Thus, the material in this book will emphasize recent advances in knowledge rather than methodological aspects, except when special procedures are reviewed. Nicholas Catsimpoolas James W. Drysdale vii Contents Chapter J Isoelectric Focusing of Human Saliva, Cerebrospinal Fluid, and Urine Josie A. Beeley I. Introduction I II. Isoelectric Focusing of Saliva . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 A. Gel Rods and Thin Layer Gels . . . . . . . . . . . . . . . . . . . . . . . . 2 B. Liquid Columns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 C. a-Amylases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 D. Immunoglobulins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 E. Blood Group Specific Substances . . . . . . . . . . . . . . . . . . . . . 11 F. Vitamin B12 Binding Proteins (Cobalophilins) . . . . . . . . . . . 12 G. Other Glycoproteins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 H. Lysozyme . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 I. Specific Salivary Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 J. Dental Plaque . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 K. Other Proteins . . . . . . . . . . . . . . . , . . . . . . . . . . . . . . . . . . . . . . 16 III. Isoelectric Focusing of Cerebrospinal Fluid . . . . . . . . . . . . . . . .

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  • Isoelectric Focusing of Human Saliva, Cerebrospinal Fluid, and Urine

    Josie A. Beeley

    Pages 1-28

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eBook $69.99 net
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  • ISBN 978-1-4613-4181-9
  • digitally watermarked, no DRM
  • included format: PDF
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Softcover $99.00 net
( price for USA )
  • ISBN 978-1-4613-4183-3
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  • usually dispatched within 3 to 5 business days

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Bibliographic Information

Bibliographic Information
Book Title
Biological and Biomedical Applications of Isoelectric Focusing
Series Title
Biological Separations
Copyright
1977
Publisher
Springer US
Copyright Holder
Plenum Press, New York
eBook ISBN
978-1-4613-4181-9
DOI
10.1007/978-1-4613-4181-9
Softcover ISBN
978-1-4613-4183-3
Edition Number
1
Topics