Methods in Molecular Biology

In Situ Detection of DNA Damage

Methods and Protocols

Editors: Didenko, Vladimir V. (Ed.)

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  • ISBN 978-0-89603-952-0
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About this book

Detection and analysis of DNA damage is critically important in the study of the widest variety of biological phenomena, ranging from apoptosis and aging to heart diseases and anticancer drug development. In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and show how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. In-depth protocols for single-cell electrophoresis (comet assay) are presented, as well as those for the detection of indirect markers of DNA damage and modified bases. Technical reviews discuss specificity, sensitivity, advantages, and limitations of the techniques described, and compare them with alternative approaches.
State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying not only the in situ detection of DNA damage but also the labeling of apoptotic cells.

Reviews

"Investigators working in the general area of DNA damage, including apoptosis, will find this book of use. The protocols are sufficiently detailed so as to allow experienced laboratory workers access to the described methods. . .the broad spectrum of methods described allows for individual investigators to exercise options."-Doody's Health Sciences Book Review Journal

"Here, a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa)." - European Journal of Histochemistry

Table of contents (22 chapters)

  • Labeling DNA Damage with Terminal Transferase

    Walker, P. Roy (et al.)

    Pages 3-19

  • TUNEL Assay

    Loo, Deryk T.

    Pages 21-30

  • Electron Microscopic Detection of DNA Damage Labeled by TUNEL

    Migheli, Antonio

    Pages 31-39

  • Quantitative Differentiation of Both Free 3′ OH and 5′ OH DNA Ends Using Terminal Transferase-Based Labeling Combined with Transmission Electron Microscopy

    Otsuki, Yoshinori (et al.)

    Pages 41-54

  • Determination of Three-Dimensional Distribution of Apoptotic DNA Damage by Combination of TUNEL and Quick-Freezing and Deep-Etching Techniques

    Ohno, Shinichi (et al.)

    Pages 55-68

Buy this book

Hardcover $169.00
price for USA
  • ISBN 978-0-89603-952-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
  • ISBN 978-1-61737-272-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
In Situ Detection of DNA Damage
Book Subtitle
Methods and Protocols
Editors
  • Vladimir V. Didenko
Series Title
Methods in Molecular Biology
Series Volume
203
Copyright
2002
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
Hardcover ISBN
978-0-89603-952-0
Softcover ISBN
978-1-61737-272-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVII, 313
Number of Illustrations and Tables
133 b/w illustrations, 31 illustrations in colour
Topics