Methods in Molecular Biology

Capillary Electrophoresis of Nucleic Acids

Editors: Mitchelson, Keith R., Cheng, Jing (Eds.)

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About this book

The advent of capillary electrophoresis (CE) , with its power to separate and analyze very small amounts of DNA, has produced a host of DNA analytical procedures. In the
Unprecedentedly wide-ranging volumes of Capillary Electrophoresis of Nucleic Acids, an outstanding panel of hands-on experts and developers of CE equipment describe in step-by-step fashion their best cutting-edge methods for the detection and analysis of DNA mutations and modifications, ranging from precise DNA loci to entire genomes of organisms. This second volume of the set, Practical Applications of Capillary Electrophoresis, covers techniques for high-throughput analysis of DNA fragments using SNP detection, mutation detection, DNA sequencing methods, and DNA-ligand interactions. Several chapters also discuss CE analysis at elevated temperatures and the use of micro-CE and array-CE devices. The companion volume, Introduction to the Capillary Electrophoresis of Nucleic Acids, offers readily reproducible methods for the analysis of small oligonucleotides and modified nucleotides, and time-tested advice on instrumentation, signal detection, the capillary environment, and the integration of mass spectrometry with CE.
Comprehensive and up-to-date, the paired volumes of Capillary Electrophoresis of Nucleic Acids offer an authoritative guide with easy access to fast, versatile, reliable, and powerful technologies for all those basic and clinical investigators analyzing DNA variation today.

Reviews

"These volumes comprehensively and accurately present CE technology and techniques with an emphasis on providing an understanding of the theory and instrumentation (Vol. 1), as well as detailed examples of successful analytical protocols (both volumes)....Chapters on the use of microchip and mass spectrometric detection are also included....Although numerous experimental protocols can be found in Vol. 1, it is clear that the intent is to communicate the underlying theory and capability of CE for separations. Vol. 1 should be of interest to the novice as well as the expert. In Vol. 2, each of the 31 chapters present specific examples of the use of CE. ...Nonetheless, these two volumes of the Methods in Molecular Biology series offer a representative and needed overview of the current theory and practice of CE of nucleic acids. These texts should prove very useful to both active investigators in this area and those seeking to learn more about the use and and capability of CE." - Clinical Chemistry

Table of contents (31 chapters)

  • Development of a High-Throughput Capillary Electrophoresis Protocol for DNA Fragment Analysis

    Wenz, H. Michael (et al.)

    Pages 3-17

  • Ultra-Fast DNA Separations Using Capillary Electrophoresis

    Klepárník, Karel (et al.)

    Pages 19-39

  • Fast DNA Fragment Sizing in a Short Capillary Column

    Issaq, Haleem J. (et al.)

    Pages 41-45

  • Detection of DNA Polymorphisms Using PCR-RFLP and Capillary Electrophoresis

    Butler, John M. (et al.)

    Pages 49-56

  • High Resolution Analysis of Point Mutations by Constant Denaturant Capillary Electrophoresis (CDCE)

    Khrapko, Konstantin (et al.)

    Pages 57-72

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-59259-116-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.00
price for USA
  • ISBN 978-0-89603-765-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $199.00
price for USA
  • ISBN 978-1-61737-177-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Capillary Electrophoresis of Nucleic Acids
Editors
  • Keith R. Mitchelson
  • Jing Cheng
Series Title
Methods in Molecular Biology
Series Volume
163
Copyright
2001
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-116-9
DOI
10.1385/1592591167
Hardcover ISBN
978-0-89603-765-6
Softcover ISBN
978-1-61737-177-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XX, 408
Topics