Methods in Molecular Biology

DNA Sequencing Protocols

Editors: Graham, Colin A, Hill, Alison J.M. (Eds.)

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About this book

Since the publication of the first edition of DNA Sequencing Protocols in 1993, major advances in its technology have made PCR-based semiautomated fluorescent sequencing the norm and enabled the complete sequencing of complex genomes. In DNA Sequencing Protocols, 2nd Edn., Colin Graham and a team of leading investigators and expert clinical scientists update the first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. Each method provides detailed, step-by-step instructions to ensure successful results, and includes helpful notes identifying and addressing potential problems. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project.
State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will impact today's genome sequencing projects, improve the accuracy and quality of DNA sequences obtained by smaller laboratories, and help lay the foundation for molecular diagnostics.

Reviews

"DNA Sequencing Protocols covers the commonly used protocols in a clearly written manner and is thorough in description. This book is highly recommended for all libraries serving researchers involved in any aspect of DNA sequencing." - E-STREAMS

Buy this book

Softcover $149.00
price for USA
  • ISBN 978-1-61737-150-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA
  • ISBN 978-0-89603-721-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.

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Bibliographic Information

Bibliographic Information
Book Title
DNA Sequencing Protocols
Editors
  • Colin A Graham
  • Alison J.M. Hill
Series Title
Methods in Molecular Biology
Series Volume
167
Copyright
2001
Publisher
Humana Press
Copyright Holder
Humana Press
Softcover ISBN
978-1-61737-150-9
Softcover ISBN
978-0-89603-721-2
Series ISSN
1064-3745
Edition Number
2
Number of Pages
256
Topics