Methods in Molecular Biology

2-D Proteome Analysis Protocols

Editors: Link, Andrew J. (Ed.)

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About this book

In 2-D Gel Proteome Analysis Protocols, Andrew Link and his expert collaborators take today's researchers step-by-step through the complete process of doing proteomics. With easy-to-follow instructions, complete with many helpful hints and explanations, leading investigators and pioneers in the field show how to make protein extracts, reproducibly run them on a 2-D gels, detect them, analyze the data, and precisely identify each protein. The book covers the latest methods of using carrier ampholytes in the 1st dimension, casting and running immobilized pH gradient 2-D gels, MALDI-TOF-based peptide mapping, automated tandem mass spectrometry, and nanoelectrospray ionization technology. For the 2nd dimension, there are methods for running flatbed or vertical gels and for protein detection using autoradiography, and Coomassie, silver, and reversible metal-chelate stains. The book is a perfect complement to the genome sequencing project for answering biological questions.

2-D Gel Proteome Analysis Protocols is the most complete guide for using proteomics to answer biological questions. Whether it is a question of global protein analysis or evaluating a cell's response to internal or external stimuli, the advanced methods described here will enable today's researchers better to understand how cells work and open new possibilities for drug discovery.

Reviews

"Our readers will find in this book with its 55 chapters, written by 78 highly well-trained specialists, all they need to come to a good result, going out from the theory of the subject to the precise description of the protocols, the instrumentation, to additive notes, the problems and the eventual difficulties. In one word, an excellent and practical book."-Cellular and Molecular Biology

"...There are 55 chapters covering everything you really need to know to get you to speed in the analysis of the proteome. I really liked the layout of each chapter with a pithy introduction followed by materials and methods sections...an absolute must for the proteomic laboratory."-Microbiology Today

". . .the book covers practical aspects , is well documented, and clearly written . . . recommended for researchers in the field. This collection of protocols is an invaluable starting point for people embarking on attempts to develop new proteomes."-FEBS Letters

"The wealth of techniques and helpful hints presented in this book should be useful to both neophytes and advanced researchers engaged in the area of high resolution 2-D protein electrophoresis."-Analytical Biochemistry

Table of contents (56 chapters)

  • 2-D Protein Gel Electrophoresis

    Fichmann, Jenny (et al.)

    Pages 1-7

  • Solubilization of Proteins in 2-D Electrophoresis

    Rabilloud, Thierry

    Pages 9-19

  • Preparation of Escherichia coli Samples for 2-D Gel Analysis

    VanBogelen, Ruth A. (et al.)

    Pages 21-29

  • Preparing 2-D Protein Extracts from Yeast

    Schieltz, David M.

    Pages 31-34

  • 2-D Protein Extracts from Drosophila melanogaster

    Ericsson, Christer

    Pages 35-41

Buy this book

eBook $159.00
price for USA (gross)
  • ISBN 978-1-59259-584-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $209.00
price for USA
  • ISBN 978-0-89603-524-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $209.00
price for USA
  • ISBN 978-1-61737-060-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
2-D Proteome Analysis Protocols
Editors
  • Andrew J. Link
Series Title
Methods in Molecular Biology
Series Volume
112
Copyright
1999
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-584-6
DOI
10.1385/1592595847
Hardcover ISBN
978-0-89603-524-9
Softcover ISBN
978-1-61737-060-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVII, 601
Topics