Techniques in Quantification and Localization of Gene Expression

Editors: Patterson, Bruce K. (Ed.)

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About this book

Can the son or daughter of a baseball pitcher or cricket bowler throw a ball 100 miles an hour? Is the son or daughter of an opera singer also an opera singer? Is a house with functional light switches lit? The line of thinking in these rhetorical questions also applies to human genetics. What do baseball pitchers, opera sing­ ers, light switches, and the Human Genome Project have in common? These questions address the issue of potential versus realization of function. Although sons and daughters of baseball pitchers and opera singers may have inherited the mechanical attributes to be baseball pitchers and opera singers, they may not, at any point in time, be baseball pitchers or opera singers. A house with functional light switches is not lit unless the light switches are on. Similarly, all of the genes discovered and sequenced as a result of the Human Genome Project are not expressed at the same time. Genome project information will allow us to deter­ mine the repertoire of genes in an individual, which is analogous to determining where the light switches in a house are located and whether they are functional (a mutation or deletion in the Genome Project Model). The pattern of "on" light switches in a house gives us functional information as to what the family inside is doing (e. g. , eating, reading, sleeping). Similarly, the pattern of gene expression (RNA) gives us information on what our bodies are doing (e. g.

Table of contents (11 chapters)

  • Basic Flow Cytometry Instrumentation

    Goolsby, Charles L. (et al.)

    Pages 3-22

  • Fixation for In Situ Molecular Analysis

    Patterson, Bruce K. (et al.)

    Pages 23-34

  • A Method for Reduction of Green Wavelength Autofluorescence Emission in Cellular Preparations

    Mosiman, Victoria L.

    Pages 35-43

  • Gene Quantification: Choosing the Target

    Patterson, Bruce K.

    Pages 45-53

  • Detection and Quantification of Cytokine-Producing Cells by Immunostaining

    Andersson, Ulf (et al.)

    Pages 55-79

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-4612-1342-0
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.00
price for USA
  • ISBN 978-0-8176-4034-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $189.00
price for USA
  • ISBN 978-1-4612-7103-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Techniques in Quantification and Localization of Gene Expression
Editors
  • Bruce K. Patterson
Copyright
2000
Publisher
Birkhäuser Basel
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4612-1342-0
DOI
10.1007/978-1-4612-1342-0
Hardcover ISBN
978-0-8176-4034-7
Softcover ISBN
978-1-4612-7103-1
Edition Number
1
Number of Pages
XVII, 150
Topics