Focus on Structural Biology

Mass Spectrometry and Genomic Analysis

Editors: Housby, J.N. (Ed.)

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About this book

76 2. Short Oligonucleotide Mass Analysis 76 2. 1. Method Outline 76 2. 2. Design of PCR Primers and Fragments for Analysis 78 2. 3. Typical PCR Reaction Conditions 79 3. Electrospray Ionisation Mass Spectrometry 79 Formation of Ions 3. 1. 79 3. 2. Tandem Mass Spectrometry 79 3. 3. Typical ESI-MS Settings for SOMA 80 4. Purification Procedures 80 4. 1. Phenol/Chloroform Extraction and Ethanol Precipitation 80 4. 2. In-line HPLC Purification 81 5. Genotyping Using SOMA 81 5. 1. APC Genotyping in Human Subjects 81 5. 2. APC Genotyping in Min Mice 85 5. Mutation Detection Using SOMA 86 6. 1. Analysis of p53 Mutations in Liver Cancer Patients 86 6. 1. 1. p53 Mutations in Liver Tumours 87 6. 1. 2. p53 Mutations in Plasma Samples 88 7. Advantages and Disadvantages of SOMA 89 8. Future Perspectives 90 9. Acknowledgements 91 10. References 91 CHAPTER 7 WV. Bienvenut, M. Müller, PM. Palagi, E. Gasteiger, M. Heller, E. Jung, M. Giron, R. Gras, S. Gay, PA. Binz, G J. Hughes, JC. Sanchez, RD. Appel, DF. Hochstrasser Proteomics and Mass Spectrometry: Some Aspects and Recent Developments 1. Introduction to Proteomics 93 2. Protein Biochemical and Chemical Processing Followed by Mass Spectrometric Analysis 94 2. 1. 2-DE Gel Protein Separation 95 Protein Identification Using Peptide Mass Fingerprinting and Robots 96 2. 2. 2. 2. 1. MALDI-MS Analysis 98 2. 2. 2. MS/MS Analysis 102 Improvement of the Identification by Chemical Modification of Peptides 106 2. 2. 3.

Table of contents (7 chapters)

  • Single-nucleotide Polymorphism Analysis by MALDI-TOF Mass Spectrometry

    Griffin, T. J. (et al.)

    Pages 1-15

  • SNP Genotyping by MALDI-TOF Mass Spectrometry

    Haff, L. A. (et al.)

    Pages 16-32

  • Massarray™: Highly Accurate and Versatile High Throughput Analysis of Genetic Variations

    Köster, Hubert

    Pages 33-49

  • The Good Assay

    Sauer, S. (et al.)

    Pages 50-65

  • Microchip Analysis of DNA Sequence by Contiguous Stacking of Oligonucleotides and Mass Spectrometry

    Tsatsos, P. H. (et al.)

    Pages 66-75

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-0-306-47595-5
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $209.00
price for USA
  • ISBN 978-0-7923-7173-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $189.00
price for USA
  • ISBN 978-94-017-3909-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Mass Spectrometry and Genomic Analysis
Editors
  • J.N. Housby
Series Title
Focus on Structural Biology
Series Volume
2
Copyright
2001
Publisher
Springer Netherlands
Copyright Holder
Springer Science+Business Media Dordrecht
eBook ISBN
978-0-306-47595-5
DOI
10.1007/0-306-47595-2
Hardcover ISBN
978-0-7923-7173-1
Softcover ISBN
978-94-017-3909-2
Series ISSN
1571-4853
Edition Number
1
Number of Pages
XV, 149
Topics