Cell Engineering

Cell Engineering

Transient Expression

Editors: Al-Rubeai, Mohamed (Ed.)

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About this book

The advantages of the baculovirus system are rooted in the properties of the virus and the host (insect, or cell lines derived from it). During the normal infection cycle, two forms of the virus are produced: an early budded virus (BY) form (Kost et al. , 2000), in which the viral DNA and structural proteins are surrounded by membrane derived from the infected cell; and a late occluded form (occlusion-derived virus, ODy), consisting of enveloped viral cores which are embedded in a crystal matrix of viral proteins. The principal component of the matrix is the abundantly expressed protein polyhedrin. The budded virus rapidly spreads the infection from cell to cell within the insect host, resulting ultimately in the complete liquefaction of the host, and release of occluded virus into the environment. The occluded form protects the released virus, allowing it to survive for long periods in the environment until ingested by another host. In the alkaline environment ofthe insect gut, the protective protein matrix is removed, and the life cycle is repeated. In insect cell cultures, only the BV form of baculovirus is required, and the polyhedrin gene may be replaced with the gene for the recombinant protein. An additional benefit of replacing or deleting polyhedrin is that it effectively makes the virus unable to survive outside the laboratory, an advantage in terms of environmental safety. The system is intrinsically safe to animals, being unable to replicate in species other than a limited range of insects.

Table of contents (8 chapters)

  • Application of Recombinant Baculoviruses in Biopharmaceutical Research

    Kost, T. A. (et al.)

    Pages 1-28

  • Engineering Post-Translational Processing of Recombinant Proteins Produced in Insect Cell Culture

    Ailor, E. (et al.)

    Pages 29-42

  • A Guide to Successful Scale-up of the Baculovirus Expression System

    Mannix, C. (et al.)

    Pages 43-55

  • Transient Gene Expression in Mammalian Cells Based on the Calcium Phosphate Transfection Method

    Jordan, M.

    Pages 56-79

  • Adenovirus Vectors in Functional Genomics

    Oualikene, Wahiba (et al.)

    Pages 80-154

Buy this book

eBook $169.00
price for USA (gross)
  • ISBN 978-94-011-4315-8
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $289.00
price for USA
  • ISBN 978-0-7923-6596-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $219.00
price for USA
  • ISBN 978-94-010-5866-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Cell Engineering
Book Subtitle
Transient Expression
Editors
  • Mohamed Al-Rubeai
Series Title
Cell Engineering
Series Volume
2
Copyright
2000
Publisher
Springer Netherlands
Copyright Holder
Kluwer Academic Publishers
eBook ISBN
978-94-011-4315-8
DOI
10.1007/978-94-011-4315-8
Hardcover ISBN
978-0-7923-6596-9
Softcover ISBN
978-94-010-5866-7
Series ISSN
1389-6946
Edition Number
1
Number of Pages
VII, 223
Topics