Pathogen and Microbial Contamination Management in Micropropagation

1. Front Matter

   Pages i-xii

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2. Overview

   1. Pathogen and Microbial Contamination Management in Micropropagation — An Overview

      • Alan C. Cassells

      Pages 1-13

   2. Strategic Considerations for the Establishment of Micro-Organism-Free Tissue Cultures for Commercial Ornamental Micropropagation

      • D. P. Holdgate, E. A. Zandvoort

      Pages 15-22

   3. Improved Virus Detection in Rosaceous Fruit Trees in vitro

      • E. Knapp, V. Hanzer, D. Mendonça, A. da Câmara Machado, H. Katinger, M. Laimer da Câmara Machado

      Pages 23-29

   4. Trends in European Plant Tissue Culture Industry from 1990 to 1993

      • F. Ó. Ríordáin

      Pages 31-37

   5. Commercial Micropropagation Laboratories in the United States

      • Richard H. Zimmerman

      Pages 39-44

3. Pathogen and Contaminant Detection and Identification

   1. Walled and Wall-Less Eubacteria from Plants: Sieve-Tube-Restricted Plant Pathogens

      • J. M. Bové, Monique Garnier

      Pages 45-60

   2. Modern Methods for Identifying Bacteria

      • David E. Stead, Judy Hennessy, Judith Wilson

      Pages 61-73

   3. Developments in Serological Methods to Detect and Identify Plant Viruses

      • L. Torrance

      Pages 75-82

   4. Molecular Diagnostics for Pathogen Detection in Seeds and Planting Material

      • J. C. Reeves

      Pages 83-95

   5. Isolation and Analysis of Double-Stranded RNA in Plant Tissues to Detect Virus and Virus-Like Agents

      • A. Teifion Jones

      Pages 97-105

   6. A Review of the lux-Marker System: Potential for Application in Plant Tissue Culture

      • Duncan White, Ken Killham, Carlo Leifert

      Pages 107-113

   7. DNA Profiling and Strategies for in Planta Localisation of Bacteria intimately Associated with Billbergia Magnifica ssp. Acutisepalia

      • Georgios Tsoktouridis, Sinclair Mantell, Eleni Bantinaki, Madan Thangavelu

      Pages 115-122

   8. Cost Analysis of Detection of Bacteria and Phytoplasmas in Plant Tissue Cultures by PCR

      • Susan Seal

      Pages 123-130

   9. Microbes Intimately Associated with Tissue and Cell Cultures of Tropical Dioscorea Yams

      • Sinclair H. Mantell

      Pages 131-138

   10. Detection and Identification of Bacterial Contaminants of Strawberry Runner Explants

       • Piyarak Tanprasert, Barbara M. Reed

       Pages 139-143

   11. Evidence for the Occurrence of Endophytic Prokaryotic Contaminants in Micropropagated Plantlets of Prunus Cerasus cv. ‘Montmorency’

       • R. Kamoun, P. Lepoivre, P. Boxus

       Pages 145-148

   12. Occurrence and Influence of Endogenous Bacteria in Embryogenic Cultures of Norway Spruce

       • D. Ewald, G. Naujoks, I. Zaspel, K. Szczygiel

       Pages 149-154

4. Chemotherapy and Thermotherapy of Plants and Cultures

   1. Antibiotics in Plant Tissue Culture and Micropropagation — What are We Aiming at?

      • Frederick R. Falkiner

      Pages 155-160

   2. Elimination of External and internal Contaminants in Rhizomes of Zantedeschia Aethiopica with Commercial Fungicides and Antibiotics

      • E. M. Kritzinger, R. Jansen van Vuuren, B. Woodward, I. H. Rong, M. H. Spreeth, M. M. Slabbert

      Pages 161-167

This book is based mainly on invited and offered papers presented at the Second International Symposium on Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures held at University College, Cork, Ireland in September 1996, with additional invited papers. The First International Symposium on Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures was held at the same venue in 1987 and was published as Acta Horticulturae volume 225, 1988. In the intervening years there have been considerable advances in both plant disease diagnostics and in the development of structured approaches to the management of disease and microbial contamination in micropropagation. These approaches have centred on attempts to separate, spatially, the problems of disease transmission and laboratory contamination. Disease-control is best achieved by establishing pathogen-free cultures while laboratory contamination is based on subsequent good working practice. Control of losses due to pathogens and microbial contamination in vitro addresses, arguably, the most importance causes of losses in the industry; nevertheless, losses at and post establishment can also be considerable due to poor quality microplants or micro-shoots. In this symposium, a holistic approach to pathogen and microbial contamination control is evident with the recognition that micropropagators must address pathogen and microbial contamination in vitro, and diseases and microplant failure at establishment. There is increasing interest in establishing beneficial bacterial and mycorrhizal association with microplants in vitro and in vivo.

• bacteria
• development
• mycorrhiza
• plant
• plants
• taxonomy
• tissue

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