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Protein Purification

Principles and Practice

  • Textbook
  • © 1994

Overview

Authors:
  1. Robert K. Scopes

    1. Department of Biochemistry and the Centre for Protein and Enzyme Technology, La Trobe University, Bundoora, Australia

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Part of the book series: Springer Advanced Texts in Chemistry (SATC)

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Table of contents (12 chapters)

  1. Front Matter

    Pages i-xix
    Download chapter PDF

  2. The Protein Purification Laboratory

    • Robert K. Scopes
    Pages 1-21

  3. Making an Extract

    • Robert K. Scopes
    Pages 22-43

  4. Analysis—Measurement of Protein and Enzyme Activity

    • Robert K. Scopes
    Pages 44-70

  5. Separation by Precipitation

    • Robert K. Scopes
    Pages 71-101

  6. Separation by Adsorption I: General Principles

    • Robert K. Scopes
    Pages 102-145

  7. Separation by Adsorption II: Ion Exchangers and Nonspecific Adsorbents

    • Robert K. Scopes
    Pages 146-186

  8. Separation by Adsorption—Affinity Techniques

    • Robert K. Scopes
    Pages 187-237

  9. Separation in Solution

    • Robert K. Scopes
    Pages 238-269

  10. Purification of Special Types of Proteins

    • Robert K. Scopes
    Pages 270-282

  11. Small-Scale and Large-Scale Procedures

    • Robert K. Scopes
    Pages 283-292

  12. Analysis for Purity

    • Robert K. Scopes
    Pages 293-309

  13. Optimization of Procedures; Final Steps

    • Robert K. Scopes
    Pages 310-345

  14. Back Matter

    Pages 346-380
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Keywords

About this book

New textbooks at all levels of chemistry appear with great regularity. Some fields such as basic biochemistry, organic re action mechanisms, and chemical thermodynamics are weil represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research that is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instruc­ tive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In so me cases the availability of texts in active research areas should help stimulate the creation of new courses.

Reviews

From reviews of earlier editions: "good practical advice that is presented in a pleasantly readable form" (Analytical Biochemistry), "well organized and written clearly" (American Scientist), "should be on every laboratory shelf where protein are being handled or purified...a feast and a genuine pleasure to read" (Nature)

 

Authors and Affiliations

  • Department of Biochemistry and the Centre for Protein and Enzyme Technology, La Trobe University, Bundoora, Australia

    Robert K. Scopes

Bibliographic Information

  • Book Title: Protein Purification

  • Book Subtitle: Principles and Practice

  • Authors: Robert K. Scopes

  • Series Title: Springer Advanced Texts in Chemistry

  • DOI: https://doi.org/10.1007/978-1-4757-2333-5

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag New York 1994

  • Hardcover ISBN: 978-0-387-94072-4Published: 19 November 1993

  • Softcover ISBN: 978-1-4419-2833-7Published: 06 December 2010

  • eBook ISBN: 978-1-4757-2333-5Published: 29 June 2013

  • Series ISSN: 0172-6323

  • Edition Number: 3

  • Number of Pages: XX, 380

  • Topics: Biochemistry, general, Cell Biology

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