Handbook of Single-Molecule Biophysics

Editors: Hinterdorfer, Peter, van Oijen, Antoine (Eds.)

  • Describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping
  • Addresses the use of single-molecule techniques in super-resolution and functional imaging
  • Includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells
  • Integrates single-molecule biophysics and nanoscience
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eBook $229.00
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  • ISBN 978-0-387-76497-9
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Hardcover $299.00
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About this book

The last decade has seen the development of a number of novel biophysical methods that allow the manipulation and study of individual biomolecules. The ability to monitor biological processes at this fundamental level of sensitivity has given rise to an improved understanding of the underlying molecular mechanisms. Through the removal of ensemble averaging, distributions and fluctuations of molecular properties can be characterized, transient intermediates identified, and catalytic mechanisms elucidated. By applying forces on biomolecules while monitoring their activity, important information can be obtained on how proteins couple function to structure. The Handbook of Single-Molecule Biophysics provides an introduction to these techniques and presents an extensive discussion of the new biological insights obtained from them. Coverage includes:

  • Experimental techniques to monitor and manipulate individual biomolecules
  • The use of single-molecule techniques in super-resolution and functional imaging
  • Single-molecule studies of physical properties of biomolecules
  • Single-molecule enzymology and biochemistry
  • Single molecules in the membrane
  • Single-molecule techniques in living cells
  • Integration of single-molecule biophysics and nanoscience

Reviews

From the reviews: “The Handbook of Single-Molecule Biophysics, edited by Hinterdorfer (Johannes Kepler Univ. of Linz, Austria) and van Oijen (Harvard Medical School), contains 21 chapters that address various aspects of techniques used to investigate single-molecule biophysics. The chapters, many authored by leading experts in their respective fields, cover specialized biophysical techniques, their applications, background into the technical design, and practical aspects of implementing these approaches. … Summing Up: Recommended. Graduate students, researchers, faculty, and professionals.” (C. A. Reinhart-King, Choice, Vol. 47 (10), June, 2010)

Table of contents (21 chapters)

  • Single-Molecule Fluorescent Particle Tracking

    Yildiz, Ahmet

    Pages 1-18

  • Single-Molecule Analysis of Biomembranes

    Schmidt, Thomas (et al.)

    Pages 19-42

  • Single-Molecule Imaging in Live Cells

    Xiao, Jie

    Pages 43-93

  • Fluorescence Imaging at Sub-Diffraction-Limit Resolution with Stochastic Optical Reconstruction Microscopy

    Dempsey, Graham T. (et al.)

    Pages 95-127

  • Single-Molecule FRET: Methods and Biological Applications

    Hwang, Ling Chin (et al.)

    Pages 129-163

Buy this book

eBook $229.00
price for USA (gross)
  • ISBN 978-0-387-76497-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $299.00
price for USA
  • ISBN 978-0-387-76496-2
  • Free shipping for individuals worldwide
  • Online orders shipping within 2-3 days.
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Bibliographic Information

Bibliographic Information
Book Title
Handbook of Single-Molecule Biophysics
Editors
  • Peter Hinterdorfer
  • Antoine van Oijen
Copyright
2009
Publisher
Springer-Verlag New York
Copyright Holder
Springer-Verlag New York
eBook ISBN
978-0-387-76497-9
DOI
10.1007/978-0-387-76497-9
Hardcover ISBN
978-0-387-76496-2
Edition Number
1
Number of Pages
XXI, 626
Topics