Photoaffinity Labeling for Structural Probing Within Protein

1. Front Matter

   Pages i-viii

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2. Multifunctional Photoprobes for Identification of Ligand Sites Within Biomolecules

   • Makoto Hashimoto

   Pages 1-11

3. Tag-Creation Approaches for Highly Efficient Profiling of Interacting Proteins and Domains

   • Takenori Tomohiro

   Pages 13-43

4. Recent Developments in Designing Compact Biological Photoprobes

   • Amberlyn M. Wands, Jennifer J. Kohler

   Pages 45-78

5. Utilities of Anti-ligand Antibody and Mass Spectrum to Elucidate Photolabeled Sites

   • Kohichi Kawahara, Katsuyoshi Masuda, Masaji Ishiguro, Hitoshi Nakayama

   Pages 79-92

6. Combination of Photoaffinity Label and Site-Directed Antibody for Target Proteins

   • Akihiko Kuniyasu, Hitoshi Nakayama

   Pages 93-110

7. Synthesis of Diazirinyl Photophore and Optically Pure Diazirinylphenylalanines for Photoaffinity Labeling

   • Yuta Murai, Lei Wang, Makoto Hashimoto

   Pages 111-128

8. Photoreactive Biomacromolecules: Installation of Photoreactive Units and Applications for Analyzing Biological Interfaces

   • Yutaka Sadakane, Isao Ohtsuka, Yasumaru Hatanaka

   Pages 129-157

9. Covalently Capturing Protein Interactions in Living Cells by Site-Specific Incorporation of Photo-Cross-Linkable Amino Acids

   • Nobumasa Hino, Kensaku Sakamoto

   Pages 159-181

10. Use of Hexose Photolabels to Reveal the Structure and Function of Glucose Transporters

    • Geoffrey D. Holman

    Pages 183-196

11. Function of Peroxisome in Mammal and Analysis of the Fatty Acid Oxidation System by Photoaffinity Labeling

    • Tsuneo Imanaka, Kosuke Kawaguchi, Masashi Morita

    Pages 197-223

12. Photoaffinity Labeling Methods to Explore Internalization Mechanisms of Arginine-Rich Cell-Penetrating Peptides

    • Gen Tanaka, Yoshimasa Kawaguchi, Keiko Kuwata, Toshihide Takeuchi, Ikuhiko Nakase, Shiroh Futaki

    Pages 225-240

13. Photoaffinity Labeling in Drug Discovery Research

    • Hideyuki Muranaka, Takaki Momose, Chiaki Handa, Tomonaga Ozawa

    Pages 241-265

This book covers the most up-to-date photoaffinity labeling method to tackle the key loop module involved in the binding process of a bioactive small molecule to its host protein. The book introduces rational points for preparing powerful photoaffinity probes, keys for the efficient analysis of labeled products, and recent successful applications for protein probing. Regarding drug design, the unique topics of the book are the special consideration of the crosslinking potential of recent probes and their application of important receptor proteins . This book presents emerging technologies of photoaffinity labeling to readers who are working in the fields of proteomics, molecular recognition, and drug discovery and development.

• Protein analysis
• Affinity-based profiling
• Membrane protein
• Bioactive molecule
• Ligand receptor
• Molecular recognition
• Binding site
• Photoactivatable probe
• Multifunctional probe
• Diazirine
• Carbene
• Cross-linking
• Drug discovery
• Chemical biology
• biochemical engineering

• University of Toyama, Toyama, Japan

  Yasumaru Hatanaka

• Graduate School of Agriculture, Hokkaido University, Sapporo, Japan

  Makoto Hashimoto

​Yasumaru Hatanaka, PhD
Executive Vice President, University of ToyamaToyama, Japan


Makoto Hashimoto, PhD
Graduate School of Agriculture, Hokkaido University
Sapporo, Japan

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