Skip to main content
  • Book
  • © 2019

Organoids

Stem Cells, Structure, and Function

Editors:

  • Includes cutting-edge methods and protocols for the study of organoids
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1576)

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 139.00
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (30 protocols)

  1. Front Matter

    Pages i-xv
  2. A Simple Method of Generating 3D Brain Organoids Using Standard Laboratory Equipment

    • Magdalena Sutcliffe, Madeline A. Lancaster
    Pages 1-12
  3. Organoid Culture of Human Cancer Stem Cells

    • Yohei Shimono, Junko Mukohyama, Taichi Isobe, Darius M. Johnston, Piero Dalerba, Akira Suzuki
    Pages 23-31
  4. Construction of Thymus Organoids from Decellularized Thymus Scaffolds

    • Asako Tajima, Isha Pradhan, Xuehui Geng, Massimo Trucco, Yong Fan
    Pages 33-42
  5. Expansion of Human Airway Basal Stem Cells and Their Differentiation as 3D Tracheospheres

    • Robert E. Hynds, Colin R. Butler, Sam M. Janes, Adam Giangreco
    Pages 43-53
  6. Generation of Functional Kidney Organoids In Vivo Starting from a Single-Cell Suspension

    • Valentina Benedetti, Valerio Brizi, Christodoulos Xinaris
    Pages 101-112
  7. Isolation and Culture of Adult Intestinal, Gastric, and Liver Organoids for Cre-recombinase-Mediated Gene Deletion

    • Dustin J. Flanagan, Renate H. M. Schwab, Bang M. Tran, Toby J. Phesse, Elizabeth Vincan
    Pages 123-133
  8. The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine

    • Malgorzata Pierzchalska, Malgorzata Panek, Malgorzata Czyrnek, Maja Grabacka
    Pages 135-144
  9. Derivation of Intestinal Organoids from Human Induced Pluripotent Stem Cells for Use as an Infection System

    • Jessica L. Forbester, Nicholas Hannan, Ludovic Vallier, Gordon Dougan
    Pages 157-169
  10. Murine Colonic Organoid Culture System and Downstream Assay Applications

    • Yang-Yi Fan, Laurie A. Davidson, Robert S. Chapkin
    Pages 171-181
  11. Intestinal Organoids as a Novel Tool to Study Microbes–Epithelium Interactions

    • Giulia Nigro, Melissa Hanson, Cindy Fevre, Marc Lecuit, Philippe J. Sansonetti
    Pages 183-194
  12. Oncogenic Transformation of Human-Derived Gastric Organoids

    • Nina Bertaux-Skeirik, Jomaris Centeno, Jian Gao, Joel Gabre, Yana Zavros
    Pages 205-213
  13. Human Intestinal Enteroids: New Models to Study Gastrointestinal Virus Infections

    • Winnie Y. Zou, Sarah E. Blutt, Sue E. Crawford, Khalil Ettayebi, Xi-Lei Zeng, Kapil Saxena et al.
    Pages 229-247

About this book

This detailed volume addresses the challenge of how to instruct stem/early progenitor cells to progress through appropriate steps to generate functional 3-dimensional organs, one of the outstanding issues in regenerative medicine. The field of organoids is geared towards defining and demonstrating the in vitro conditions that achieve this goal. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and cutting-edge, Organoids: Stem Cells, Structure, and Function serves as an aid to researchers working in this vital area of research.

Editors and Affiliations

  • Ottawa Hospital Research Institute, Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Organoids

  • Book Subtitle: Stem Cells, Structure, and Function

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7617-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-7616-4Published: 13 September 2019

  • eBook ISBN: 978-1-4939-7617-1Published: 12 September 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 363

  • Number of Illustrations: 23 b/w illustrations, 49 illustrations in colour

  • Topics: Cell Biology

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 139.00
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access