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Noncanonical Amino Acids

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1728)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xi
  2. Engineering in the Test Tube

    1. Front Matter

      Pages 1-1
    2. Tub-Tag Labeling; Chemoenzymatic Incorporation of Unnatural Amino Acids

      • Jonas Helma, Heinrich Leonhardt, Christian P. R. Hackenberger, Dominik Schumacher
      Pages 67-93
  3. Engineering in Eukaryotes

    1. Front Matter

      Pages 219-219
    2. Generation of Stable Amber Suppression Cell Lines

      • Simon J. Elsässer
      Pages 237-245
    3. Trapping Chromatin Interacting Proteins with Genetically Encoded, UV-Activatable Crosslinkers In Vivo

      • Christian Hoffmann, Heinz Neumann, Petra Neumann-Staubitz
      Pages 247-262

About this book

This volume covers some of the most widely used protocols on nanocanonical amino acids, providing details and advice for users to get each method up and running for their chosen application.  Chapters have been divided into three parts describing methods for protein production in the test tube, in prokaryotes, and in eukaryotes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Noncanonical Amino Acids: Methods and Protocols aims to provide readers with techniques that enable them to design new experiments and create new areas of research.

Editors and Affiliations

  • Structural and Computational Biology Unit & Cell Biology and Biophysics Unit, EMBL, Heidelberg, Germany

    Edward A. Lemke

Bibliographic Information

  • Book Title: Noncanonical Amino Acids

  • Book Subtitle: Methods and Protocols

  • Editors: Edward A. Lemke

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7574-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7573-0Published: 06 February 2018

  • Softcover ISBN: 978-1-4939-8525-8Published: 04 June 2019

  • eBook ISBN: 978-1-4939-7574-7Published: 05 February 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 411

  • Number of Illustrations: 37 b/w illustrations, 39 illustrations in colour

  • Topics: Biochemistry, general

Buy it now

Buying options

eBook USD 79.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 99.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 139.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access