Overview
- Editors:
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Stephen D. Skaper
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Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy
- Includes cutting-edge methods and protocols for the study of neurotrophic factors
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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Table of contents (36 protocols)
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- Paola Tirassa, Pamela Rosso, Angela Iannitelli
Pages 19-38
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- Laura Facci, Stephen D. Skaper
Pages 39-47
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- Stephen D. Skaper, Laura Facci
Pages 49-61
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- Stephen D. Skaper, Laura Facci
Pages 63-80
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- Christine A. Webber, Douglas W. Zochodne
Pages 81-92
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- Aviva M. Tolkovsky, Jack Brelstaff
Pages 93-105
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- Stephen D. Skaper, Massimo Barbierato, Vanni Ferrari, Morena Zusso, Laura Facci
Pages 107-118
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- Paola Brun, Hamid I. Akbarali
Pages 119-125
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- Laura Facci, Massimo Barbierato, Stephen D. Skaper
Pages 127-137
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- Cinzia Volonté, Adele De Ninno, Susanna Amadio
Pages 139-154
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- Massimo Barbierato, Stephen D. Skaper, Laura Facci
Pages 155-166
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- Laura Facci, Stephen D. Skaper
Pages 167-178
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- M. Foster Olive, Armani P. Del Franco, Cassandra D. Gipson
Pages 179-200
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- Dimitar Dimitrov, Laurent Guillaud, Kohgaku Eguchi, Tomoyuki Takahashi
Pages 201-215
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- Matias Alloatti, Luciana Bruno, Tomas L. Falzone
Pages 217-226
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- Nicholas Radio, Steven Frank
Pages 227-237
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- Philip Lazarovici, Adi Lahiani, Galit Gincberg, Dikla Haham, Arnon Fluksman, Ofra Benny et al.
Pages 239-250
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- Philip Lazarovici, Adi Lahiani, Galit Gincberg, Dikla Haham, Cezary Marcinkiewicz, Peter I. Lelkes
Pages 251-259
About this book
This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. It begins with chapters on the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia and glia-glia co-culture models, oligodendrocytes, and cell-based assays for the evaluation of cell vitality. This revised second edition expands to cover methodology encompassing site-specific direct labeling of neurotrophins and their receptors, angiogenesis assays, stem cells, pancreatic beta-cells, axonal transport, synapse biology, dendritic spine analysis, and brain endothelial cells. Subsequent chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides, as well as protocols describing nanofiber- and nanoparticle-based methods for brain delivery of neurotrophic agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Â
Authoritative and practical, Neurotrophic Factors: Methods and Protocols, Second Edition is a valuable resource for a wide audience of readers as they explore nervous system function and pathology.
Editors and Affiliations
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Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy
Stephen D. Skaper