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  • © 2018

Maize

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1676)

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Table of contents (13 protocols)

  1. Front Matter

    Pages i-xi
  2. Cell, Tissue, and Organ Culture and Maize Transformation

    1. Front Matter

      Pages 1-1
    2. Agrobacterium- and Biolistic-Mediated Transformation of Maize B104 Inbred

      • Jennifer A. Raji, Bronwyn Frame, Daniel Little, Tri Joko Santoso, Kan Wang
      Pages 15-40
    3. Advances in Agrobacterium-mediated Maize Transformation

      • Heng Zhong, Sivamani Elumalai, Samson Nalapalli, Lee Richbourg, Anna Prairie, David Bradley et al.
      Pages 41-59
  3. Gene Silencing and Generation of Mutant Populations

    1. Front Matter

      Pages 95-95
    2. Deletion Mutagenesis and Identification of Causative Mutations in Maize

      • Shangang Jia, Aixia Li, Chi Zhang, David Holding
      Pages 97-108
    3. Selective Knockdowns in Maize by Sequence-Specific Protein Aggregation

      • Camilla Betti, Joost Schymkowitz, Frederic Rousseau, Eugenia Russinova
      Pages 109-127
    4. Use of a Virus Gene Silencing Vector for Maize Functional Genomics Research

      • Tao Zhou, Xuedong Liu, Zaifeng Fan
      Pages 141-150
  4. Plant Gene Expression

    1. Front Matter

      Pages 151-151
    2. Ribosome Profiling in Maize

      • Prakitchai Chotewutmontri, Nicholas Stiffler, Kenneth P. Watkins, Alice Barkan
      Pages 165-183
    3. Detection of DNA Methylation by Whole-Genome Bisulfite Sequencing

      • Qing Li, Peter J. Hermanson, Nathan M. Springer
      Pages 185-196
    4. Plant Gene Regulation Using Multiplex CRISPR-dCas9 Artificial Transcription Factors

      • Levi G. Lowder, Aimee Malzahn, Yiping Qi
      Pages 197-214
  5. Plant Metabolic Networks

    1. Front Matter

      Pages 215-215
    2. Cellular and Subcellular Level Localization of Maize Lipids and Metabolites Using High-Spatial Resolution MALDI Mass Spectrometry Imaging

      • Maria Emilia Dueñas, Adam D. Feenstra, Andrew R. Korte, Paige Hinners, Young Jin Lee
      Pages 217-231
  6. Back Matter

    Pages 233-234

About this book

This volume discusses techniques used for the molecular characterization of maize. This book is divided into 4 parts: cell, tissue, and organ culture and maize transformation; gene silencing and generation of mutant populations; plant gene expression; and plant metabolic networks. The chapters cover a range of topics, such as growing and propagating maize in the laboratory, greenhouse and field studies, screening mutagenic population, characterizing the genome, describing protein and metabolic regulatory networks, and generating transgenic plants for gene knock-out and over expression purposes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Maize: Methods and Protocols is a valuable resource for everyone who is interested in maize research.

Editors and Affiliations

  • Department of Agronomy and Horticulture, University of Nebraska – Lincoln, Lincoln, USA

    L. Mark Lagrimini

Bibliographic Information

  • Book Title: Maize

  • Book Subtitle: Methods and Protocols

  • Editors: L. Mark Lagrimini

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7315-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7314-9Published: 07 October 2017

  • Softcover ISBN: 978-1-4939-8450-3Published: 14 August 2018

  • eBook ISBN: 978-1-4939-7315-6Published: 07 October 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 234

  • Number of Illustrations: 6 b/w illustrations, 24 illustrations in colour

  • Topics: Plant Sciences

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access