A Beginner’s Guide to Microarrays

1. Front Matter

   Pages i-xvi

   PDF

2. Slide Coating and DNA Immobilization Chemistries

   • Kathryn Aboytes, Jason Humphreys, Sonya Reis, Brian Ward

   Pages 1-41

3. Diagnostic Oligonucleotide Microarrays for Microbiology

   • Levente Bodrossy

   Pages 43-91

4. Printing Technologies and Microarray Manufacturing Techniques: Making the Perfect Microarray

   • Todd Martinsky

   Pages 93-122

5. Arrays for the Masses—Setting up a Microarray Core Facility

   • Robert P. Searles

   Pages 123-149

6. Microarray Data Normalization: The Art and Science of Overcoming Technical Variance to Maximize the Detection of Biologic Variance

   • Maureen A. Sartor, Mario Medvedovic, Bruce J. Aronow

   Pages 151-178

7. Experimental Design and Data Analysis

   • Eric Blalock

   Pages 179-241

8. Microarray Experiment Design and Statistical Analysis

   • Xuejun Peng, Arnold J. Stromberg

   Pages 243-275

9. Strategies for Clustering, Classifying, Integrating, Standardizing and Visualizing Microarray Gene Expression Data

   • Willy Valdivia Granda

   Pages 277-340

10. Back Matter

    Pages 341-347

    PDF

A Beginner's Guide to Microarrays addresses two audiences - the core facility manager who produces, hybridizes, and scans arrays, and the basic research scientist who will be performing the analysis and interpreting the results. User friendly coverage and detailed protocols are provided for the technical steps and procedures involved in many facets of microarray technology, including:

-Cleaning and coating glass slides,
-Designing oligonucleotide probes,
-Constructing arrays for the detection and quantification of different bacterial species,
-Preparing spotting solutions,
-Troubleshooting spotting problems,
-Setting up and running a core facility,
-Normalizing background signal and controlling for systematic variance,
-Designing experiments for maximum effect,
-Analyzing data with statistical procedures,
-Clustering data with machine-learning protocols. 

• DNA
• Nucleotide
• RNA
• clustering
• data analysis
• database
• gene expression
• genes
• performance
• Tree Biology

From the reviews:

"The book offers a broad coverage of many of the most important aspects of microarray technology … . Despite the wide field broached by this book, one of its chief successes lies with the fact that in each chapter the authors provide a clear review of every step of the technique, including both its potential and pitfalls. This allows all readers, especially beginners, to understand the development and impact of this important technique. … Readers will find this beginner’s guide book very informative … ." (Guillermina Lopez-Bendito, Journal of Chemical Neuroanatomy, Vol. 30, 2005)

• University of Kentucky Medical Center, USA

  Eric M. Blalock

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